Rapid detection of gyrA and parC mutations in quinolone-resistant Salmonella enterica using Pyrosequencing® technology
- 31 January 2007
- journal article
- research article
- Published by Elsevier BV in Journal of Microbiological Methods
- Vol. 68 (1), 163-171
- https://doi.org/10.1016/j.mimet.2006.07.006
Abstract
Fluoroquinolones are broad-spectrum antimicrobials highly effective in the treatment of a wide variety of clinical infections. Salmonella gastroenteritis is usually only treated with fluoroquinolones when the patient is elderly or immunocompromised. Fluoroquinolones are also used for the treatment of systemic Salmonella infection or for long-term salmonella carriage. Resistance to quinolones is commonly mediated by point mutations within the topoisomerase genes gyrA and parC. Pyrosequencing® technology is a DNA sequencing method using ‘sequencing by synthesis’ and is suitable for the rapid detection of single nucleotide polymorphisms (SNPs).Keywords
This publication has 23 references indexed in Scilit:
- Mechanisms of quinolone resistance in Escherichia coli and Salmonella: Recent developmentsInternational Journal of Antimicrobial Agents, 2005
- Prevalence of Mutations within the Quinolone Resistance-Determining Region of gyrA , gyrB , parC , and parE and Association with Antibiotic Resistance in Quinolone-Resistant Salmonella entericaAntimicrobial Agents and Chemotherapy, 2004
- Role of TolC and parC mutation in high-level fluoroquinolone resistance in Salmonella enterica serotype Typhimurium DT204Journal of Antimicrobial Chemotherapy, 2004
- Topoisomerase IV Mutations in Quinolone-Resistant Salmonellae SelectedIn VitroMicrobial Drug Resistance, 2003
- Phenotypic and Genotypic Characterization of Food Animal Isolates ofSalmonellawith Reduced Sensitivity to CiprofloxacinMicrobial Drug Resistance, 2002
- Detection of gyrA Mutations in Quinolone-Resistant Salmonella enterica by Denaturing High-Performance Liquid ChromatographyJournal of Clinical Microbiology, 2002
- Comparative Studies of Mutations in Animal Isolates and Experimental In Vitro- and In Vivo-Selected Mutants of Salmonella spp. Suggest a Counterselection of Highly Fluoroquinolone-Resistant Strains in the FieldAntimicrobial Agents and Chemotherapy, 1999
- Development of quinolone resistance in Salmonella: Failure to prevent splenic abscessJournal of Infection, 1997
- Topoisomerase IV is a target of quinolones in Escherichia coli.Proceedings of the National Academy of Sciences of the United States of America, 1995
- High-level fluoroquinolone resistance in a Salmonella typhimurium isolate due to alterations in both gyrA and gyrB genesJournal of Antimicrobial Chemotherapy, 1993