Human peritoneal macrophages show functional characteristics of M‐CSF‐driven anti‐inflammatory type 2 macrophages
- 24 May 2007
- journal article
- research article
- Published by Wiley in European Journal of Immunology
- Vol. 37 (6), 1594-1599
- https://doi.org/10.1002/eji.200737042
Abstract
We have recently shown that in vitro polarized M-CSF-driven anti-inflammatory macrophages (MΦ2) have the unique capacity to preferentially bind and ingest early apoptotic cells. However, these data are based on in vitro polarized cells and it is unclear whether MΦ2-like cells exist in vivo. Here we used CD163 as a cell surface marker to distinguish MΦ2 from the pro-inflammatory MΦ1. We show that human peritoneal MΦ (pMΦ) freshly isolated from patients on peritoneal dialysis have the phenotypical characteristics of MΦ2, including CD163 surface expression and lack of CD16. Like MΦ2, pMΦ have the capacity for endocytosis and macropinocytosis, are able to preferentially bind and ingest early apoptotic cells, and produce large amounts of IL-10 upon stimulation with LPS. Moreover, upon LPS stimulation both pMΦ and MΦ2 down-regulate CD86, resulting in a reduced capacity to stimulate proliferation of allogeneic T cells and an inhibition of Th1 cytokine release of these T cells. Our data provide the evidence for the first time that in vitro polarized MΦ2 exist in vivo, and human pMΦ resemble the anti-inflammatory MΦ2. We propose that pMΦ have the potential to maintain an anti-inflammatory condition in the peritoneal cavity.Keywords
This publication has 22 references indexed in Scilit:
- Dendritic cell and macrophage subsets in the handling of dying cellsImmunobiology, 2006
- IL-10–producing macrophages preferentially clear early apoptotic cellsBlood, 2006
- AM-3K, an Anti-macrophage Antibody, Recognizes CD163, a Molecule Associated with an Anti-inflammatory Macrophage PhenotypeJournal of Histochemistry & Cytochemistry, 2006
- MACROPHAGE RECEPTORS AND IMMUNE RECOGNITIONAnnual Review of Immunology, 2005
- A blast from the past: clearance of apoptotic cells regulates immune responsesNature Reviews Immunology, 2002
- Apoptotic cell removalCurrent Biology, 2001
- Human resident peritoneal macrophages: phenotype and biologyBritish Journal of Haematology, 1994
- Antigen-presenting capacity of macrophages and dendritic cells in the peritoneal cavity of patients treated with peritoneal dialysisClinical and Experimental Immunology, 1993
- Inhibition of the immunosuppressive activity of resident pulmonary alveolar macrophages by granulocyte/macrophage colony-stimulating factor.The Journal of Experimental Medicine, 1993
- Downregulation of the antigen presenting cell function(s) of pulmonary dendritic cells in vivo by resident alveolar macrophages.The Journal of Experimental Medicine, 1993