Development and Evaluation of Novel Real-Time Reverse Transcription-PCR Assays with Locked Nucleic Acid Probes Targeting Leader Sequences of Human-Pathogenic Coronaviruses
- 1 August 2015
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 53 (8), 2722-2726
- https://doi.org/10.1128/jcm.01224-15
Abstract
Based on findings in small RNA-sequencing (Seq) data analysis, we developed highly sensitive and specific real-time reverse transcription (RT)-PCR assays with locked nucleic acid probes targeting the abundantly expressed leader sequences of Middle East respiratory syndrome coronavirus (MERS-CoV) and other human coronaviruses. Analytical and clinical evaluations showed their noninferiority to a commercial multiplex PCR test for the detection of these coronaviruses.Keywords
This publication has 41 references indexed in Scilit:
- The structure and functions of coronavirus genomic 3′ and 5′ endsVirus Research, 2015
- Development and Validation of a Rapid Immunochromatographic Assay for Detection of Middle East Respiratory Syndrome Coronavirus Antigen in Dromedary CamelsJournal of Clinical Microbiology, 2015
- A Systematic Approach to Novel Virus Discovery in Emerging Infectious Disease OutbreaksThe Journal of Molecular Diagnostics, 2015
- SARS Coronavirus Detection MethodsEmerging Infectious Diseases, 2005
- Antigenic Cross‐Reactivity between Severe Acute Respiratory Syndrome–Associated Coronavirus and Human Coronaviruses 229E and OC43The Journal of Infectious Diseases, 2005
- Characterization and Complete Genome Sequence of a Novel Coronavirus, Coronavirus HKU1, from Patients with PneumoniaJournal of Virology, 2005
- Reverse Transcriptase PCR Diagnostic Assay for the Coronavirus Associated with Severe Acute Respiratory SyndromeJournal of Clinical Microbiology, 2004
- Genome structure and transcriptional regulation of human coronavirus NL63Virology Journal, 2004
- LNA: a versatile tool for therapeutics and genomicsTrends in Biotechnology, 2003
- Molecular analysis of the human coronavirus (strain 229E) genomeArchives of Virology. Supplementum, 1993