Immunomagnetic separation and detection of Salmonella cells using newly designed carriers
- 1 August 2003
- journal article
- Published by Elsevier BV in Journal of Chromatography A
- Vol. 1009 (1-2), 215-221
- https://doi.org/10.1016/s0021-9673(03)00431-x
Abstract
Magnetic nonporous poly(HEMA-co-EDMA) and poly(HEMA-co-GMA) microspheres were prepared by dispersion copolymerisation of 2-hydroxyethyl methacrylate (HEMA) and ethylene dimethacrylate (EDMA) or glycidyl methacrylate (GMA) in the presence of magnetite. They were functionalized by polyclonal Salmonella antibodies via the trichlorotriazine method. Salmonella cells were then successfully identified using cultural and polymerase chain reaction (PCR) methods after their immunomagnetic separation. The PCR sensitivity of target cell detection was negatively influenced by the presence of some compounds used in the process of particle preparation. In some cases, magnetic poly(HEMA-co-EDMA) microspheres with immobilized proteinase K were used for degradation of intracellular inhibitors present in Salmonella cells.Keywords
This publication has 17 references indexed in Scilit:
- Salmonella surveillance: a global survey of public health serotypingEpidemiology and Infection, 2002
- Characterization of deoxyribonuclease I immobilized on magnetic hydrophilic polymer particlesJournal of Chromatography B, 2002
- Properties of RNase A Immobilized on Magnetic Poly(2‐hydroxyethyl methacrylate) MicrospheresBiotechnology Progress, 2001
- PCR identification of Salmonella cells in food and stool samples after immunomagnetic separationBioseparation, 2000
- Oriented immobilization of biologically active proteins as a tool for revealing protein interactions and functionJournal of Chromatography B: Biomedical Sciences and Applications, 1999
- Rapid detection of stressed Salmonella spp. in dairy and egg products using immunomagnetic separation and PCRInternational Journal of Food Microbiology, 1999
- Oriented immobilization of restriction endonuclease ecoriJournal of Molecular Recognition, 1996
- Hydroxyethyl methacrylate-based sorbents for high-performance liquid chromatography of proteinsJournal of Chromatography A, 1994
- Salmonella identification by the polymerase chain reactionMolecular and Cellular Probes, 1993
- Immobilized NucleasesCritical Reviews in Biotechnology, 1993