Two classes of short interfering RNA in RNA silencing
Top Cited Papers
- 2 September 2002
- journal article
- Published by Springer Science and Business Media LLC in The EMBO Journal
- Vol. 21 (17), 4671-4679
- https://doi.org/10.1093/emboj/cdf464
Abstract
RNA silencing is a eukaryotic genome defence system that involves processing of double‐stranded RNA (dsRNA) into 21–26 nt, short interfering RNA (siRNA). The siRNA mediates suppression of genes corresponding to the dsRNA through targeted RNA degradation. In some plant systems there are additional silencing processes, involving systemic spread of silencing and RNA‐directed methylation/transcriptional suppression of homologous genomic DNA. We show here that siRNAs produced in plants from a green fluorescent protein (GFP) transgene are in short (21–22 nt) and long (24–26 nt) size classes, whereas those from endogenous retroelements are only in the long class. Viral suppressors of RNA silencing and mutations in Arabidopsis indicate that these classes of siRNA have different roles. The long siRNA is dispensable for sequence‐specific mRNA degradation, but correlates with systemic silencing and methylation of homologous DNA. Conversely, the short siRNA class correlates with mRNA degradation but not with systemic signalling or methylation. These findings reveal an unexpected level of complexity in the RNA silencing pathway in plants that may also apply in animals.Keywords
This publication has 62 references indexed in Scilit:
- Functional anatomy of siRNAs for mediating efficient RNAi in Drosophila melanogaster embryo lysateThe EMBO Journal, 2001
- On the Role of RNA Amplification in dsRNA-Triggered Gene SilencingCell, 2001
- A Cellular Function for the RNA-Interference Enzyme Dicer in the Maturation of the let-7 Small Temporal RNAScience, 2001
- Genes and Mechanisms Related to RNA Interference Regulate Expression of the Small Temporal RNAs that Control C. elegans Developmental TimingCell, 2001
- Mobilization of transposons by a mutation abolishing full DNA methylation in ArabidopsisNature, 2001
- HC-Pro Suppression of Transgene Silencing Eliminates the Small RNAs but Not Transgene Methylation or the Mobile SignalTHE PLANT CELL ONLINE, 2001
- RNAi: Double-Stranded RNA Directs the ATP-Dependent Cleavage of mRNA at 21 to 23 Nucleotide IntervalsCell, 2000
- Silencing of Retrotransposons in Arabidopsis and Reactivation by the ddm1 MutationTHE PLANT CELL ONLINE, 2000
- RNA–DNA Interactions and DNA Methylation in Post-Transcriptional Gene SilencingTHE PLANT CELL ONLINE, 1999
- Suppression of Virus Accumulation in Transgenic Plants Exhibiting Silencing of Nuclear Genes.THE PLANT CELL ONLINE, 1996