Monitoring and screening plant populations with combined thermal and chlorophyll fluorescence imaging
Open Access
- 22 December 2006
- journal article
- review article
- Published by Oxford University Press (OUP) in Journal of Experimental Botany
- Vol. 58 (4), 773-784
- https://doi.org/10.1093/jxb/erl257
Abstract
Thermal and chlorophyll fluorescence imaging are powerful tools for the study of spatial and temporal heterogeneity of leaf transpiration and photosynthetic performance. The relative advantages and disadvantages of these techniques are discussed. When combined, they can highlight pre-symptomatic responses not yet apparent in visual spectrum images and provide specific signatures for diagnosis of distinct diseases and abiotic stresses. In addition, their use for diagnosis and for selection for stomatal or photosynthetic mutants, these techniques can be applied for stress tolerance screening. For example, rapid screening for stomatal responses can be achieved by thermal imaging, while, combined with fluorescence imaging to study photosynthesis, they can potentially be used to derive leaf water use efficiency as a screening parameter. A particular advantage of imaging is that it allows continuous automated monitoring of dynamic spatial variation. Examples of applications include the study of growth and development of plant lines differing in stress resistance, yield, circadian clock-controlled responses, and the possible interactions between these parameters. In the future, such dual-imaging systems could be extended with complementary techniques such as hyperspectral and blue-green fluorescence imaging. This would result in an increased number of quantified parameters which will increase the power of stress diagnosis and the potential for screening of stress-tolerant genotypes.Keywords
This publication has 98 references indexed in Scilit:
- Optimizing thermal imaging as a technique for detecting stomatal closure induced by drought stress under greenhouse conditionsPhysiologia Plantarum, 2006
- Plant Circadian RhythmsTHE PLANT CELL ONLINE, 2006
- The differential effects of herbivory by first and fourth instars of Trichoplusia ni (Lepidoptera: Noctuidae) on photosynthesis in Arabidopsis thalianaJournal of Experimental Botany, 2005
- Estimation of leaf water potential by thermal imagery and spatial analysis*Journal of Experimental Botany, 2005
- The genetics of adaptive responses to drought stress: abscisic acid‐dependent and abscisic acid‐independent signalling componentsPhysiologia Plantarum, 2005
- Thermal and Chlorophyll-Fluorescence Imaging Distinguish Plant-Pathogen Interactions at an Early StagePlant and Cell Physiology, 2004
- Safeguarding production—losses in major crops and the role of crop protectionCrop Protection, 2004
- Using multidirectional thermography to characterize water status of cottonRemote Sensing of Environment, 2003
- Inhibition of photosynthesis by Colletotrichum lindemuthianum in bean leaves determined by chlorophyll fluorescence imagingPlant, Cell & Environment, 2001
- High resolution imaging of photosynthetic activities of tissues, cells and chloroplasts in leavesJournal of Experimental Botany, 2001