Photoacoustic Imaging of Mesenchymal Stem Cells in Living Mice via Silica-Coated Gold Nanorods
- 10 June 2012
- journal article
- research article
- Published by American Chemical Society (ACS) in ACS Nano
- Vol. 6 (7), 5920-5930
- https://doi.org/10.1021/nn302042y
Abstract
Improved imaging modalities are critically needed for optimizing stem cell therapy. Techniques with real-time content to guide and quantitate cell implantation are especially important in applications such as musculoskeletal regenerative medicine. Here, we report the use of silica-coated gold nanorods as a contrast agent for photoacoustic imaging and quantitation of mesenchymal stem cells in rodent muscle tissue. The silica coating increased the uptake of gold into the cell more than 5-fold, yet no toxicity or proliferation changes were observed in cells loaded with this contrast agent. Pluripotency of the cells was retained, and secretome analysis indicated that only IL-6 was disregulated more than 2-fold from a pool of 26 cytokines. The low background of the technique allowed imaging of down to 100 000 cells in vivo. The spatial resolution is 340 μm, and the temporal resolution is 0.2 s, which is at least an order of magnitude below existing cell imaging approaches. This approach has significant advantages over traditional cell imaging techniques like positron emission tomography and magnetic resonance imaging including real time monitoring of stem cell therapy.Keywords
This publication has 62 references indexed in Scilit:
- Affibody‐Functionalized Gold–Silica Nanoparticles for Raman Molecular Imaging of the Epidermal Growth Factor ReceptorSmall, 2011
- Silica-Coated Gold Nanorods as Photoacoustic Signal NanoamplifiersNano Letters, 2011
- Design, Synthesis, and Imaging of an Activatable Photoacoustic ProbeJournal of the American Chemical Society, 2010
- Ultrahigh Sensitivity Carbon Nanotube Agents for Photoacoustic Molecular Imaging in Living MiceNano Letters, 2010
- Photoacoustic imaging of living mouse brain vasculature using hollow gold nanospheresBiomaterials, 2010
- Golden carbon nanotubes as multimodal photoacoustic and photothermal high-contrast molecular agentsNature Nanotechnology, 2009
- Mesenchymal Stem Cells: Emerging Therapy for Duchenne Muscular DystrophyPM&R, 2009
- Muscular Dystrophy Therapy by Nonautologous Mesenchymal Stem Cells: Muscle Regeneration Without Immunosuppression and InflammationTransplantation, 2009
- Carbon nanotubes as photoacoustic molecular imaging agents in living miceNature Nanotechnology, 2008
- Pluripotency of mesenchymal stem cells derived from adult marrowNature, 2002