Biodiversity inventories in high gear: DNA barcoding facilitates a rapid biotic survey of a temperate nature reserve
Open Access
- 30 August 2015
- journal article
- Published by Pensoft Publishers in Biodiversity Data Journal
- Vol. 3 (3), e6313
- https://doi.org/10.3897/bdj.3.e6313
Abstract
The existing species inventory for the rare Charitable Research Reserve was rapidly expanded by integrating a DNA barcoding workflow with two surveying strategies - a comprehensive sampling scheme over four months, followed by a one-day bioblitz involving international taxonomic experts. The two surveys resulted in 25,287 and 3,502 specimens barcoded, respectively, as well as 127 human observations. This barcoded material, all vouchered at the Biodiversity Institute of Ontario collection, covers 14 phyla, 29 classes, 117 orders, and 531 families of animals, plants, fungi, and lichens. Overall, the ATBI documented 1,102 new species records for the nature reserve, expanding the existing long-term inventory by 49%. In addition, 2,793 distinct Barcode Index Numbers (BINs) were assigned to genus or higher level taxonomy, and represent additional species that will be added once their taxonomy is resolved. For the 3,502 specimens, the collection, sequence analysis, taxonomic assignment, data release and manuscript submission by 100+ co-authors all occurred in less than one week. This demonstrates the speed at which barcode-assisted inventories can be completed and the utility that barcoding provides in minimizing and guiding valuable taxonomic specialist time. The final product is more than a comprehensive biotic inventory - it is also a rich dataset of fine-scale occurrence and sequence data, all archived and cross-linked in the major biodiversity data repositories. This model of rapid generation and dissemination of essential biodiversity data could be followed to conduct regional assessments of biodiversity status and change, and potentially be employed for evaluating progress towards the Aichi Targets of the Strategic Plan for Biodiversity 2011-2020.Keywords
This publication has 43 references indexed in Scilit:
- A DNA-Based Registry for All Animal Species: The Barcode Index Number (BIN) SystemPLOS ONE, 2013
- Revealing the Hyperdiverse Mite Fauna of Subarctic Canada through DNA BarcodingPLOS ONE, 2012
- DNA Barcoding Methods for Land PlantsPublished by Springer Science and Business Media LLC ,2012
- Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for FungiProceedings of the National Academy of Sciences of the United States of America, 2012
- Refining the DNA barcode for land plantsProceedings of the National Academy of Sciences of the United States of America, 2011
- The adventive genus Xantholinus Dejean (Coleoptera, Staphylinidae, Staphylininae in North America: new records and a synthesis of distributional dataZooKeys, 2010
- In the dark in a large urban park: DNA barcodes illuminate cryptic and introduced moth speciesBiodiversity and Conservation, 2009
- DNA barcoding and the mediocrity of morphologyMolecular Ecology Resources, 2009
- Towards a comprehensive barcode library for arctic life - Ephemeroptera, Plecoptera, and Trichoptera of Churchill, Manitoba, CanadaFrontiers in Zoology, 2009
- bold: The Barcode of Life Data System (http://www.barcodinglife.org)Molecular Ecology Notes, 2007