In vitro Mass Multiplication with Pure Genetic Identity in Anthurium andreanum Lind.

Abstract

A novel protocol for enhanced in vitro multiple shoot induction was developed for Anthurium andreanum Lind. First shoot bud was induced within a week in MS supplemented with 0.1 mg/l NAA and 0.25 mg/l BAP where maximum six buds from single apical bud explant appeared within next 20 days. For multiple shoot proliferation MS with 0.5 mg/l BAP and 60 mg/l adenine sulphate (ADS) proved to be best resulting ten multiple shoots per inoculated shoot bud within 50 days. As many as 11 roots per plantlet were produced in 27 days using MS with 0.5 mg/l IAA and 2 g/l activated charcoal. Autoclaved sand and intermittent water spraying optimized the primary hardening period of 15 days and then earthen pots filled with sand, soil, charcoal and coconut fibre ensured the recovery of 51 well hardened plantlets out of 60 in next 30 days showing 85% success. The genetic identity of both ex vitro hardened clones and in vitro sustained clones with their mother ant were tested using 10 ISSR primers, displayed no polymorphism. Key words: Anthurium, In vitro cloning, Multiple shoot, Genetic identity D.O.I. 10.3329/ptcb.v18i2.3361 Plant Tissue Cult. & Biotech. 18(2): 113-122, 2008 (December)