Sample preparation for polar metabolites in bioanalysis
- 27 November 2017
- journal article
- review article
- Published by Royal Society of Chemistry (RSC) in The Analyst
- Vol. 143 (1), 16-20
- https://doi.org/10.1039/c7an01333g
Abstract
Sample preparation is a primary step of any bioanalytical workflow, especially in metabolomics analysis where maximum information has to be obtained without spoiling the analytical instrument. Because of their biological implication, highly polar metabolites, such as amino acids, nucleobases, and catecholamines seem to attract growing interest in the field of comprehensive metabolomics analysis although their extraction from the matrix remains a real challenge. In this paper, we discuss about the actual practice and issues of hydrophilic metabolites' extraction, including new solutions and perspectives to improve their phase transfer from a complex biological sample to a clean extract prior to analysis.Keywords
This publication has 47 references indexed in Scilit:
- Exhaustive extraction of peptides by electromembrane extractionAnalytica Chimica Acta, 2015
- ‘Dilute-and-Shoot’ RSLC–MS-MS Method for Fast Detection of Nerve and Vesicant Chemical Warfare Agent Metabolites in UrineJournal of Analytical Toxicology, 2014
- Evaluation of human plasma sample preparation protocols for untargeted metabolic profiles analyzed by UHPLC-ESI-TOF-MSAnalytical and Bioanalytical Chemistry, 2014
- Current advances and strategies towards fully automated sample preparation for regulated LC–MS/MS bioanalysisBioanalysis, 2014
- Electromembrane extraction—Three‐phase electrophoresis for future preparative applicationsElectrophoresis, 2014
- Parallel electromembrane extraction in the 96-well formatAnalytica Chimica Acta, 2014
- Optimization of solid phase microextraction coatings for liquid chromatography mass spectrometry determination of neurotransmittersJournal of Chromatography A, 2014
- Sample preparation and orthogonal chromatography for broad polarity range plasma metabolomics: Application to human subjects with neurodegenerative dementiaAnalytical Biochemistry, 2014
- Recent developments in dispersive liquid–liquid microextractionAnalytical and Bioanalytical Chemistry, 2013
- Parallel electromembrane extraction in a multiwell plateAnalytical and Bioanalytical Chemistry, 2013