Expression of macrophage migration inhibitory factor in diffuse systemic sclerosis
Open Access
- 1 May 2003
- journal article
- other
- Published by BMJ in Annals Of The Rheumatic Diseases
- Vol. 62 (5), 460-464
- https://doi.org/10.1136/ard.62.5.460
Abstract
Objective: To evaluate whether, in patients with the diffuse form of systemic sclerosis (dSSc), macrophage migration inhibitory factor (MIF) production is dysregulated. Methods: 10 patients with dSSc and 10 healthy controls, matched for age and sex, were studied. MIF expression was evaluated by immunohistochemistry on formalin fixed skin biopsies of patients with dSSc and controls. MIF levels were assayed in the sera and in the supernatants of skin cultured fibroblasts by a colorimetric sandwich enzyme linked immunosorbent assay (ELISA). MIF concentrations in culture medium samples and in serum samples were compared by Student’s two tailed t test for unpaired data. Results: Anti-MIF antibody immunostained the basal and mainly suprabasal keratinocytes. Small perivascular clusters of infiltrating mononuclear cells were positive; scattered spindle fibroblast-like cells were immunostained in superficial and deep dermal layers. The serum concentrations of MIF in patients with dSSc (mean (SD) 10705.6 (9311) pg/ml) were significantly higher than in controls (2157.5 (1288.6) pg/ml; p=0.011); MIF levels from dSSc fibroblast cultures (mean (SD) 1.74 (0.16) ng/2×105 cells) were also significantly higher than in controls (0.6 (0.2) ng/2×105 cells; p=0.008). Conclusion: These results suggest that MIF may be involved in the amplifying proinflammatory loop leading to scleroderma tissue remodelling.Keywords
This publication has 32 references indexed in Scilit:
- Macrophage migration inhibitory factor in patients with juvenile idiopathic arthritisArthritis & Rheumatism, 2002
- Chemokine expression by systemic sclerosis fibroblasts: Abnormal regulation of monocyte chemoattractant protein 1 expressionArthritis & Rheumatism, 2001
- α-Thrombin Stimulates Expression of Macrophage Migration Inhibitory Factor in Skin FibroblastsSeminars in Thrombosis and Hemostasis, 1999
- Evidence for a role of macrophage migration inhibitory factor in psoriatic skin diseaseBritish Journal of Dermatology, 1999
- Macrophage Migration Inhibitory Factor Is an Essential Immunoregulatory Cytokine in Atopic DermatitisBiochemical and Biophysical Research Communications, 1997
- Identification of macrophage migration inhibitory factor in murine neonatal calvariae and osteoblastsImmunology, 1996
- Identification and immunohistochemical localization of macrophage migration inhibitory factor in human corneaFEBS Letters, 1996
- Identification of macrophage migration inhibitory factor (MIF) in human skin and its immunohistochemical localizationFEBS Letters, 1996
- Increased interleukin 1 receptor, type I, at messenger RNA and protein level in skin fibroblasts from patients with systemic sclerosisBiochemical and Biophysical Research Communications, 1992
- Cutaneous antigen-stimulating lymphokine production by lymphocytes of patients with progressive systemic sclerosis (scleroderma).JCI Insight, 1976