Correlation between the Presence of Virulence‐Associated Genes as Determined by PCR and Actual Virulence to Mice in Various Strains of Listeria Spp.
- 1 May 1995
- journal article
- research article
- Published by Wiley in Microbiology and Immunology
- Vol. 39 (5), 343-349
- https://doi.org/10.1111/j.1348-0421.1995.tb02211.x
Abstract
Five chromosomal genes, prfA, plcA, hlyA, mpl and plcB, are implicated in the virulence of Listeria monocytogenes and some of these genes have been used for the identification of bacteria by polymerase chain reaction (PCR). Using 6 strains of L. monocytogenes and 3 L. innocua strains, the relationship was examined between the presence of five virulence-associated genes and actual virulence to mice in terms of 50% lethal dose (LD50), bacterial viability in the organ of infected mice and the intracellular growth in cultured macrophages. None of the five genes could be amplified by PCR in all the L. innocua strains and they were actually avirulent to mice. All L. monocytogenes strains were shown to be virulent and to have intact virulence-associated genes except for the strain ATCC15313. This particular strain was revealed to be avirulent and defective in hlyA and plcA in PCR amplification. It was suggested that PCR detection of genes prfA, mpl, or plcB may not be sufficient to detect virulent strains of L. monocytogenes. It appeared that the ability to produce listeriolysin O (LLO), which is encoded by hlyA, was critical for the expression of virulence regardless of the amount of LLO produced.Keywords
This publication has 29 references indexed in Scilit:
- Coordinate regulation of virulence genes in Listeria monocytogenes requires the product of the prfA geneJournal of Bacteriology, 1992
- Epidemiology of human listeriosisClinical Microbiology Reviews, 1991
- The Type Strain(s) of Listeria monocytogenes: A Source of Continuing DifficultiesInternational Journal of Systematic and Evolutionary Microbiology, 1991
- Detection of a gene encoding a phosphatidylinositol‐specific phospholipase C that is co‐ordinately expressed with listeriolysin in Listeria monocytogenesMolecular Microbiology, 1991
- Listeria monocytogenes, a food-borne pathogen.Microbiological Reviews, 1991
- Identification of a gene that positively regulates expression of listeriolysin, the major virulence factor of listeria monocytogenes.Proceedings of the National Academy of Sciences of the United States of America, 1990
- Bacillus subtilis expressing a haemolysin gene from Listeria monocytogenes can grow in mammalian cellsNature, 1990
- Listeriosis: new findings—current concernMicrobial Pathogenesis, 1988
- Role of hemolysin for the intracellular growth of Listeria monocytogenes.The Journal of Experimental Medicine, 1988
- Tn916-induced mutations in the hemolysin determinant affecting virulence of Listeria monocytogenesJournal of Bacteriology, 1987