Gene Expression Profiles Reveal that DCN, DIO1, and DIO2 Are Underexpressed in Benign and Malignant Thyroid Tumors
- 1 March 2005
- journal article
- Published by Mary Ann Liebert Inc in Thyroid®
- Vol. 15 (3), 210-221
- https://doi.org/10.1089/thy.2005.15.210
Abstract
To investigate the molecular events involved in the pathogenesis and/or progression of thyroid tumors, we compared the gene expression profiles of three thyroid carcinoma cell lines, which represent major tumor subtypes of thyroid cancer and normal thyroid tissue. Using cDNA array methodology, we investigated the expression of 1807 open reading frame expressed sequence tags (ORESTES), selected from head and neck tumor libraries generated through the Brazilian Human Cancer Project-LICR/FAPESP. We found that 505 transcripts were differentially expressed in the thyroid carcinoma cell lines. Using a more stringent criterion, transcripts underexpressed or overexpressed more than fivefold in I of 3 or 3 of 3 carcinoma cell lines, a list of 55 ESTs were detected. Five candidate genes were further validated by quantitative polymerase chain reaction (qPCR) in an independent set of 52 thyroid tumors and 22 matched normal thyroid tissues. DCN was found underexpressed in a high percentage of the follicular thyroid adenomas, follicular thyroid carcinomas, and follicular variant of papillary thyroid carcinomas. DIO1 and DIO2 were underexpressed in nearly all papillary thyroid carcinomas. These genes not only could help to better define a tumor signature for thyroid tumors, but may, in part, also become useful as potential targets for thyroid tumor treatment.Universidade Federal de São Paulo, Dept Med, Div Endocrinol, Mol Endocrinol Lab, BR-04039032 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Pathol, BR-04039032 São Paulo, BrazilUniversidade Federal de São Paulo, Div Genet, Dept Morphol, BR-04039032 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Med, Div Endocrinol, Mol Endocrinol Lab, BR-04039032 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Pathol, BR-04039032 São Paulo, BrazilUniversidade Federal de São Paulo, Div Genet, Dept Morphol, BR-04039032 São Paulo, BrazilWeb of SciencKeywords
This publication has 55 references indexed in Scilit:
- Analysis of genetic stability at the EP300 and CREBBP loci in a panel of cancer cell linesGenes, Chromosomes and Cancer, 2003
- Pure versus follicular variant of papillary thyroid carcinomaCancer, 2003
- Frequent amplification of 8q24, 11q, 17q, and 20q‐specific genes in pancreatic cancerGenes, Chromosomes and Cancer, 2002
- Suppression of tumorigenicity by adenovirus-mediated gene transfer of decorinOncogene, 2002
- Decorin suppresses transforming growth factor-β-induced expression of plasminogen activator inhibitor-1 in human mesangial cells through a mechanism that involves Ca2+-dependent phosphorylation of Smad2 at serine-240Biochemical Journal, 2002
- Identifying Differentially Expressed Genes Associated with Metastasis of Follicular Thyroid Cancer by cDNA Expression ArrayThyroid®, 2001
- Restricted expression of cadherin-8 in segmental and functional subdivisions of the embryonic mouse brainDevelopmental Dynamics, 1997
- Mode of tumor invasion in oral squamous cell carcinoma: Improved grading based on immunohistochemical examination of extracellular matricesHead & Neck, 1996
- Identification of a 27-kilodalton protein with the properties of type I iodothyronine 5'-deiodinase in human thyroid glandJournal of Clinical Endocrinology & Metabolism, 1992
- Expression of decorin in human tissues and cell lines and defined chromosomal assignment of the gene locus (DCN)Cytogenetic and Genome Research, 1992