A fast liquid chromatographic/mass spectrometric screening method for the simultaneous detection of synthetic glucocorticoids, some stimulants, anti‐oestrogen drugs and synthetic anabolic steroids
- 25 October 2006
- journal article
- research article
- Published by Wiley in Rapid Communications in Mass Spectrometry
- Vol. 20 (22), 3465-3476
- https://doi.org/10.1002/rcm.2729
Abstract
A fast liquid chromatographic/mass spectrometric (LC/MS/MS) screening method for the detection, in urine, of synthetic glucocorticoids, stimulants (formoterol, modafinil and mesocarb), anti-oestrogens (finasteride, exemestane, anastrozole, letrozole and formestane) and synthetic anabolic steroids (stanozolol, gestrinone and tetrahydrogestrinone) is described. All these drugs (and/or their urinary metabolites) can be simultaneously extracted by a single liquid/liquid extraction step, at alkaline pH, after enzymatic hydrolysis with β-glucuronidase, and assayed in 7 min by LC/MS/MS using electrospray ionization in positive ion mode and multiple reaction monitoring as the acquisition mode. All compounds show good reproducibility of both the retention times (CV% <2%) and the relative abundances (CV% <10%). The limits of detection for the anti-oestrogens, glucocorticoids and steroids are in the range of 1–30 ng/mL, and for the stimulants are in the range of 100–200 ng/mL, thus satisfying the minimum required performance limits of the World Anti-Doping Agency. Copyright © 2006 John Wiley & Sons, Ltd.Keywords
This publication has 7 references indexed in Scilit:
- Determination of endogenous and synthetic glucocorticoids in human urine by gas chromatography–mass spectrometry following microwave-assisted derivatizationAnalytica Chimica Acta, 2003
- Method for confirmation of synthetic corticosteroids in doping urine samples by liquid chromatography–electrospray ionisation mass spectrometryJournal of Chromatography A, 2001
- Analysis of corticosteroids in equine urine by liquid chromatography–mass spectrometryJournal of Chromatography B: Biomedical Sciences and Applications, 2001
- Liquid chromatography–mass spectrometry: potential in forensic and clinical toxicologyJournal of Chromatography B: Biomedical Sciences and Applications, 1999
- High-performance liquid chromatographic analysis of corticosteroidsJournal of Chromatography B: Biomedical Sciences and Applications, 1995
- Metabolism of anabolic steroids in man: synthesis and use of reference substances for identification of anabolic steroid metabolitesAnalytica Chimica Acta, 1993
- Comparison of corticosteroid derivatives by gas chromatography-mass spectrometryAnalytical Chemistry, 1972