Microprojectile bombardment of plant tissues increases transformation frequency by Agrobacterium tumefaciens
- 1 January 1992
- journal article
- Published by Springer Science and Business Media LLC in Plant Molecular Biology
- Vol. 18 (2), 301-313
- https://doi.org/10.1007/bf00034957
Abstract
Bombardment of plant tissues with microprojectiles in an effective method of wounding to promote Agrobacterium-mediated transformation. Tobacco cv. Xanthi leaves and sunflower apical meristems were wounded by microprojectile bombardment prior to application of Agrobacterium tumefaciens strains containing genes within the T-DNA encoding GUS or NPTII. Stable kanamycin-resistant tobacco transformants were obtained using an NPTII construct from particle/plasmid, particle-wounded/Agrobacterium-treated or scalpel-wounded/Agrobacterium-treated potato leaves. Those leaves bombarded with particles suspended in TE buffer prior to Agrobacterium treatment produced at least 100 times more kanamycin-resistant colonies than leaves treated by the standard particle gun transformation protocol. In addition, large sectors of GUS expression, indicative of meristem cell transformation, were observed in plants recovered from sunflower apical explants only when the meristems were wounded first by particle bombardment prior to Agrobacterium treatment. Similar results in two different tissue types suggest that (1) particles may be used as a wounding mechanism to enhance Agrobacterium transformation frequencies, and (2) Agrobacterium mediation of stable transformation is more efficient than the analogous particle/plasmid protocol.Keywords
This publication has 37 references indexed in Scilit:
- Transformation of Maize Cells and Regeneration of Fertile Transgenic Plants.Plant Cell, 1990
- A Regulatory Gene as a Novel Visible Marker for Maize TransformationScience, 1990
- Transformation of meristematic cells in the shoot apex of cultured pea shoots byAgrobacterium tumefaciens andA. rhizogenesProtoplasma, 1989
- Cell lineage patterns in the shoot meristem of the sunflower embryo in the dry seedDevelopmental Biology, 1989
- BASIC PROCESSES UNDERLYING AGROBACTERIUM-MEDIATED DNA TRANSFER TO PLANT CELLSAnnual Review of Genetics, 1988
- The biolistic processTrends in Biotechnology, 1988
- Duplication of CaMV 35 S Promoter Sequences Creates a Strong Enhancer for Plant GenesScience, 1987
- Identification of the signal molecules produced by wounded plant cells that activate T-DNA transfer in Agrobacterium tumefaciensNature, 1985
- A binary plant vector strategy based on separation of vir- and T-region of the Agrobacterium tumefaciens Ti-plasmidNature, 1983
- Genetic analysis of crown gall: Fine structure map of the T-DNA by site-directed mutagenesisCell, 1981