An Immunoblotting Procedure for Detection of Antibodies against Bovine Leukemia Virus in Cattle
- 12 January 1992
- journal article
- research article
- Published by Wiley in Journal of Veterinary Medicine, Series B
- Vol. 39 (1-10), 48-52
- https://doi.org/10.1111/j.1439-0450.1992.tb01136.x
Abstract
A sensitive protein immunoblotting (Western blot) procedure has been developed for detecting anti-BLV antibodies in cattle sera. The antibodies against most of the major viral proteins could be detected. This procedure does not give any non-specific background staining and there is absence of any erroneous results due to utilisation of purified viral preparations. The procedure has been applied for detection of antibodies to BLV in a set of 74 sera samples and it has been compared with other commonly used serological tests like ELISA and agar gel immunodiffusion test.Keywords
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