Crystallization and preliminary X-ray crystallographic analysis of the ArsM arsenic(III)S-adenosylmethionine methyltransferase
- 26 August 2010
- journal article
- crystallization communications
- Published by International Union of Crystallography (IUCr) in Acta Crystallographica Section F Structural Biology and Crystallization Communications
- Vol. 66 (9), 1050-1052
- https://doi.org/10.1107/s1744309110027661
Abstract
Arsenic is the most ubiquitous environmental toxin and carcinogen and consequently ranks first on the Environmental Protection Agency's Superfund Priority List of Hazardous Substances. It is introduced primarily from geochemical sources and is acted on biologically, creating an arsenic biogeocycle. A common biotransformation is methylation to monomethylated, dimethylated and trimethylated species. Methylation is catalyzed by the ArsM (or AS3MT) arsenic(III)S-adenosylmethionine methyltransferase, an enzyme (EC 2.1.1.137) that is found in members of every kingdom from bacteria to humans. ArsM from the thermophilic algaCyanidioschyzonsp. 5508 was expressed, purified and crystallized. Crystals were obtained by the hanging-drop vapor-diffusion method. The crystals belonged to the monoclinic space groupC2, with unit-cell parametersa= 84.85,b= 46.89,c= 100.35 Å, β = 114.25° and one molecule in the asymmetric unit. Diffraction data were collected at the Advanced Light Source and were processed to a resolution of 1.76 Å.Keywords
This publication has 8 references indexed in Scilit:
- Biotransformation of arsenic by a Yellowstone thermoacidophilic eukaryotic algaProceedings of the National Academy of Sciences of the United States of America, 2009
- Arsenic detoxification and evolution of trimethylarsine gas by a microbial arsenite S -adenosylmethionine methyltransferaseProceedings of the National Academy of Sciences of the United States of America, 2006
- Elucidating the pathway for arsenic methylation*1Toxicology and Applied Pharmacology, 2004
- The role of biomethylation in toxicity and carcinogenicity of arsenic: a research update.Environmental Health Perspectives, 2002
- [20] Processing of X-ray diffraction data collected in oscillation modeMethods in Enzymology, 1997
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- Solvent content of protein crystalsJournal of Molecular Biology, 1968
- Biological MethylationPublished by Wiley ,1951