Potato Plants Lacking the CDSP32 Plastidic Thioredoxin Exhibit Overoxidation of the BAS1 2-Cysteine Peroxiredoxin and Increased Lipid Peroxidation in Thylakoids under Photooxidative Stress
Open Access
- 1 July 2003
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 132 (3), 1335-1343
- https://doi.org/10.1104/pp.103.021626
Abstract
The CDSP32 protein (chloroplastic drought-induced stress protein of 32 kD) is a thioredoxin participating in the defense against oxidative damage. We recently have identified in vitro the BAS1 2-Cys peroxiredoxin, a peroxide-detoxifying enzyme, as a target for CDSP32. Here, we report the characterization under stress conditions of transgenic potato (Solanum tuberosum) plants lacking CDSP32 with regard to the BAS1 redox state and the level of lipid peroxidation. Under control conditions, BAS1 is present at similar levels both in wild-type (WT) and transgenic plants. Under drought and methyl viologen treatment, CDSP32-lacking plants display, compared with WT, an increased proportion of BAS1 monomer corresponding to an overoxidized form of the protein. Leaf discs from transgenic plants treated with methyl viologen exhibit earlier degradation of BAS1 than WT plants do. Using several approaches, i.e. a probe emitting fluorescence when reacting with peroxides, high-performance liquid chromatography determination of lipid hydroxy fatty acid content, and measurement of chlorophyll thermoluminescence, we show a higher lipid peroxidation level under methyl viologen treatment in thylakoids from CDSP32-lacking plants compared with WT. These data show that CDSP32 is a critical component in the defense system against lipid peroxidation in photosynthetic membranes, likely as a physiological electron donor to the BAS1 peroxiredoxin.Keywords
This publication has 53 references indexed in Scilit:
- The Plastidic 2-Cysteine Peroxiredoxin Is a Target for a Thioredoxin Involved in the Protection of the Photosynthetic Apparatus against Oxidative DamagePlant Cell, 2002
- Identification in Saccharomyces cerevisiae of a New Stable Variant of Alkyl Hydroperoxide Reductase 1 (Ahp1) Induced by Oxidative StressJournal of Biological Chemistry, 2002
- Inactivation by gene disruption of 2‐cysteine‐peroxiredoxin in Synechocystis sp. PCC 6803 leads to increased stress sensitivityPhysiologia Plantarum, 1998
- The Origin of115–130°C Thermoluminescence Bands in Chlorophyll‐Containing MaterialPhotochemistry and Photobiology, 1998
- Characterization of Three Potato Lipoxygenases with Distinct Enzymatic Activities and Different Organ-specific and Wound-regulated Expression PatternsJournal of Biological Chemistry, 1996
- Effects of low temperature, high salinity and exogenous ABA on the synthesis of two chloroplastic drought-induced proteins in Solanum tuberosumPhysiologia Plantarum, 1996
- Lipid peroxidation in tobacco leaves treated with the elicitor cryptogein: evaluation by high-temperature thermoluminescence emission and chlorophyll fluorescenceBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1995
- H2O2 from the oxidative burst orchestrates the plant hypersensitive disease resistance responseCell, 1994
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue CulturesPhysiologia Plantarum, 1962