Bisphenol A affects glucose transport in mouse 3T3‐F442A adipocytes
- 30 January 2004
- journal article
- research article
- Published by Wiley in British Journal of Pharmacology
- Vol. 141 (2), 209-214
- https://doi.org/10.1038/sj.bjp.0705520
Abstract
Recently, environmental chemicals have appeared in daily human life, and these chemicals have been incidentally taken in by humans. The serum concentrations of some of these chemicals have been found to be associated with the onset and incidence rate of diabetes mellitus. It has been suggested that one of the environmental chemicals, bisphenol A (BPA), has hormone-like activity. It has also been demonstrated that some hormones affect insulin resistance and fat distribution in the body. To study the effects of these environmental chemicals on glucose metabolism, the effect of BPA on glucose transport in mouse 3T3-F442A adipocytes was investigated. The 3T3-F442A adipocytes were incubated with various concentrations of BPA in a medium. Deoxyglucose uptake assay was performed with and without insulin. Immunoblot analysis was performed with a glucose transporter (GLUT) 4-specific antibody and antiphosphotyrosine antibody. The BPA treatment enhanced basal and insulin-stimulated glucose uptake, and caused an increased amount of GLUT4 protein. Thus, the enhanced glucose uptake resulting from the BPA treatment was at least partially due to the increased amount of GLUT4. Tyrosine phosphorylation of insulin receptor substrate-1 with insulin stimulation was not significantly affected. In conclusion, it was demonstrated that BPA, one of the chemicals that we intake incidentally, affects the glucose transport in adipocytes, and also that the environmental chemicals may be identified as one of the environmental factors that affect diabetes and obesity.Keywords
This publication has 28 references indexed in Scilit:
- Expression of glucose transporter 4 mrna in adipose tissue- and skeletal muscle of ovariectomized rats treated with sex steroid hormonesLife Sciences, 2000
- Metabolism and Disposition of Bisphenol A in Female RatsToxicology and Applied Pharmacology, 2000
- A Synthetic Antagonist for the Peroxisome Proliferator-activated Receptor γ Inhibits Adipocyte DifferentiationPublished by Elsevier BV ,2000
- Uterotrophic activity of bisphenol A in the immature rat.Environmental Health Perspectives, 1998
- Dynamics of Insulin Signaling in 3T3-L1 AdipocytesPublished by Elsevier BV ,1998
- Estrogenicity of resin-based composites and sealants used in dentistry.Environmental Health Perspectives, 1996
- The E-SCREEN assay as a tool to identify estrogens: an update on estrogenic environmental pollutants.Environmental Health Perspectives, 1995
- TRANSCRIPTIONAL REGULATION OF GENE EXPRESSION DURING ADIPOCYTE DIFFERENTIATIONAnnual Review of Biochemistry, 1995
- Xenoestrogens released from lacquer coatings in food cans.Environmental Health Perspectives, 1995
- Regulation of insulin receptor, insulin receptor substrate-1 and phosphatidylinositol 3-kinase in 3T3-F442A adipocytes. Effects of differentiation, insulin, and dexamethasoneMolecular Endocrinology, 1994