Transport at the Air/Water Interface is the Reason for Rings in Protein Microarrays
- 9 February 2006
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 128 (9), 2768-2769
- https://doi.org/10.1021/ja057669w
Abstract
Fabricating a protein microarray involves the deposition of nanoliter droplets of solutions on a solid surface. Instead of uniform spots, one often observes ring-like structures that add to the difficulty in quantification. We show that the accumulation of proteins at the air/water interface of the nanodroplet is the reason. Transformation to a uniform spot can be achieved via the addition of competitive surfactants or the control of surface reaction kinetics.Keywords
This publication has 10 references indexed in Scilit:
- Immobilization of oriented protein molecules on poly(ethylene glycol)‐coated Si(111)Proteomics, 2004
- Methods and applications of antibody microarrays in cancer researchProteomics, 2003
- Next generation of protein microarray support materials:: Evaluation for protein and antibody microarray applicationsJournal of Chromatography A, 2003
- Peptide chips for the quantitative evaluation of protein kinase activityNature Biotechnology, 2002
- Cylinder Alignment in Annular Structures of Microphase-Separated Polystyrene-b-Poly(methyl methacrylate)Langmuir, 2000
- Orogenic Displacement of Protein from the Air/Water Interface by Competitive AdsorptionJournal of Colloid and Interface Science, 1999
- Capillary flow as the cause of ring stains from dried liquid dropsNature, 1997
- Self‐Assembly of Submicrometer Rings of Particles from Solutions of NanoparticlesAngewandte Chemie, 1997
- Porphyrin WheelsJournal of the American Chemical Society, 1996
- Differences in the structure and dynamics of the adsorbed layers in protein-stabilized model foams and emulsionsFaraday Discussions, 1994