Calcitonin levels in normal individuals with new highly sensitive chemiluminescent enzyme immunoassay
- 1 January 1998
- journal article
- research article
- Published by Wiley in Journal of Clinical Laboratory Analysis
- Vol. 12 (4), 218-222
- https://doi.org/10.1002/(sici)1098-2825(1998)12:4<218::aid-jcla5>3.0.co;2-3
Abstract
Human serum calcitonin concentration in normal individuals was measured with a new assay based on the chemiluminescent enzyme immunoassay (CLEIA) method. The CLEIA assay was highly sensitive and was able to determine a calcitonin concentration of 0.04 pg/ml as sensitivity limit at a condition of 0+3SD. With this CLEIA assay, the mean value of calcitonin in males and females was 2.26 and 1.33 pg/ml, respectively, highlighting a significant difference between genders. The mean value and range of human serum calcitonin in this assay were approximately 1/10 those reported previously in competitive radioimmunoassay (RIA) methods. Since RIAs for calcitonin showed much variability at a low concentration range due to the competitive format, they seemed to lack the necessary sensitivity to cover the normal range and appeared only useful for hyper-calcitonin phenomenon in diseases such as medullary thyroid carcinoma. The CLEIA for calcitonin provided a lower detection limit than normal range, and it can therefore be assumed that it could be applied for the measurement of hypo-calcitonin phenomena typically found in some disorders such as osteoporosis.Keywords
This publication has 8 references indexed in Scilit:
- Development of a highly sensitive enzyme immunoassay for human calcitonin using solid phase coupled with multiple antibodies.Annals of Clinical Biochemistry: International Journal of Laboratory Medicine, 1999
- Highly Sensitive Chemiluminescent Enzyme Immunoassay with Gelatin-Coated Ferrite Solid PhaseClinical Chemistry, 1994
- Diagnosis of Medullary Carcinoma of the Thyroid (MCT) by Calcitonin Assay Using Monoclonal Antibodies: Criteria for the Pentagastrin Stimulation Test in Hereditary MCTJournal of Clinical Endocrinology & Metabolism, 1990
- Construction and clinical validation of a sensitive and specific assay for serum mature calcitonin using monoclonal anti-peptide antibodiesClinica Chimica Acta; International Journal of Clinical Chemistry, 1988
- Determination of Silica-Purified Plasma Calcitonin for the Detection and Management of Medullary Thyroid Carcinoma: Comparison of Two Provocative TestsMayo Clinic Proceedings, 1987
- Estimates of Circulating Monomeric Calcitonin: Physiological Studies in Normal and Thyroidectomized Man*Journal of Clinical Endocrinology & Metabolism, 1983
- Influence of Age and Sex on Plasma Calcitonin in Human BeingsNew England Journal of Medicine, 1980
- Immunoassay of Human CalcitoninNew England Journal of Medicine, 1970