Monitoring genomic alterations with a panel of oligonucleotide probes specific for various simple repeat motifs
- 1 January 1991
- journal article
- Published by Wiley in Electrophoresis
- Vol. 12 (2-3), 186-192
- https://doi.org/10.1002/elps.1150120215
Abstract
Germline and somatic instability of the human genome was studied, using synthetic oligonucletides specific for simple repeat motifs. The following probes were used: (GTG)5, (GACA)4, (GATA)4, (CT)8, (TTAGGG)3, (GT)8, (GAA)6 and (GGAT)4. Each of them is unique with respect to the target regions recognized in the genome. Thus compilation of the various fingerprint data provides a complex map of the genome (and its deviations). While the fingerprints of differentiated somatic tissues never showed any alterations, in tumor tissues (namely gliomas) many changes could be detected. Most of the latter reflect secondary karyological aberrations. In nearly one third of the gliomas, drastically amplified and apparently monomorphic DNA fragments were identified. This marker should make it possible to deal with causal pathogenetic mechanisms as well as novel diagnostic strategies.Keywords
This publication has 42 references indexed in Scilit:
- Chromosomal organization of simple repeated DNA sequencesElectrophoresis, 1991
- Telomeres shorten during ageing of human fibroblastsNature, 1990
- Human telomeres: fusion and interstitial sitesTrends in Genetics, 1989
- MECHANISM AND DEVELOPMENTAL PROGRAM OF IMMUNOGLOBULIN GENE REARRANGEMENT IN MAMMALSAnnual Review of Genetics, 1989
- Simultaneous genetic mapping of multiple human minisatellite sequences using DNA fingerprintingGenomics, 1989
- Conservation of the human telomere sequence (TTAGGG)n among vertebrates.Proceedings of the National Academy of Sciences, 1989
- Assessment of clonality in gastrointestinal cancer by DNA fingerprinting.JCI Insight, 1988
- Optimized oligonucleotide probes for DNA fingerprintingElectrophoresis, 1988
- A Sequence in M13 Phage Detects Hypervariable Minisatellites in Human and Animal DNAScience, 1987
- TECHNOLOGICAL EXAMINATION OF LOW‐FIRED TERRACOTTA STATUES FROM AYIA IRINI, KEAArchaeometry, 1982