A DNA Vaccine Encoding Lumazine Synthase fromBrucella abortusInduces Protective Immunity in BALB/c Mice
Open Access
- 1 May 2002
- journal article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 70 (5), 2507-2511
- https://doi.org/10.1128/iai.70.5.2507-2511.2002
Abstract
This study was conducted to evaluate the immunogenicity of theBrucella abortuslumazine synthase (BLS) gene cloned into the pcDNA3 plasmid, which is driven by the cytomegalovirus promoter. Injection of plasmid DNA carrying the BLS gene (pcDNA-BLS) into BALB/c mice elicited both humoral and cellular immune responses. Antibodies to the encoded BLS included immunoglobulin G1 (IgG1) IgG2a, IgG2b, IgG3, and IgM isotypes. Animals injected with pcDNA-BLS exhibited a dominance of IgG2a over IgG1. In addition, spleen cells from vaccinated animals produced interleukin-2 and gamma interferon but not IL-10 or IL-4 after in vitro stimulation with recombinant BLS (rBLS), suggesting the induction of a Th1 response. Protection was evaluated by comparing the levels of infection in the spleens of vaccinated mice challenged withB. abortus544. Immunization with pcDNA-BLS- reduced the bacterial burden relative to those in the control groups. Mice immunized with rBLS produced a significant humoral response but did not show a specific cellular response or any protection from challenge. Altogether, these data suggest that pcDNA-BLS is a good immunogen for the production of humoral and cell-mediated responses in mice and is a candidate for use in future studies of vaccination against brucellosis.Keywords
This publication has 32 references indexed in Scilit:
- Divergence in macromolecular assembly: X-ray crystallographic structure analysis of lumazine synthase from Brucella abortusJournal of Molecular Biology, 2000
- Effects of Opsonization and Gamma Interferon on Growth ofBrucella melitensis16M in Mouse Peritoneal Macrophages In VitroInfection and Immunity, 2000
- A protein particle vaccine containing multiple malaria epitopesNature Biotechnology, 1997
- Identification of the major T-cell antigens present in the Brucella melitensis B115 protein preparation, Brucellergene OCBJournal of Medical Microbiology, 1997
- Immunogenicity and efficacy of a tuberculosis DNA vaccine encoding the components of the secreted antigen 85 complexVaccine, 1997
- Immunization of mice with recombinantVaccine, 1996
- Gene gun-based nucleic acid immunization: elicitation of humoral and cytotoxic T lymphocyte responses following epidermal delivery of nanogram quantities of DNAVaccine, 1995
- Heterologous Protection Against Influenza by Injection of DNA Encoding a Viral ProteinScience, 1993
- Protection conferred on mice by combinations of monoclonal antibodies directed against outer-membrane proteins or smooth lipopolysaccharide of BrucellaJournal of Medical Microbiology, 1992
- Isolation of biologically active ribonucleic acid from sources enriched in ribonucleaseBiochemistry, 1979