Diagnosis of Oropouche virus infection by RT‐nested‐PCR
- 29 November 2001
- journal article
- research article
- Published by Wiley in Journal of Medical Virology
- Vol. 66 (1), 139-142
- https://doi.org/10.1002/jmv.2122
Abstract
Using the RT‐PCR with primers that anneal to the 5′ and the 3′ extremities of the genome segments of bunyaviruses and internal primers that anneal to the S segment of Simbu serogroup viruses in a nested PCR it was possible to amplify the Oropouche virus (ORO) genome from the sera of three patients. These results show that this RT‐nested‐PCR is a useful tool for rapid diagnosis of Oropouche fever infections. J. Med. Virol. 66:139–142, 2002.Keywords
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