Extended-spectrum and AmpC β-lactamase-producing Escherichia coli in broilers and people living and/or working on broiler farms: prevalence, risk factors and molecular characteristics
- 30 May 2014
- journal article
- Published by Oxford University Press (OUP) in Journal of Antimicrobial Chemotherapy
- Vol. 69 (10), 2669-2675
- https://doi.org/10.1093/jac/dku178
Abstract
The objectives of this study were to: estimate the prevalence of extended-spectrum β-lactamase (ESBL)- and AmpC β-lactamase-producing Escherichia coli carriage among broiler farmers, their family members and employees; identify and quantify risk factors for carriage, with an emphasis on contact with live broilers; and compare isolates from humans and broilers within farms with respect to molecular characteristics to gain insight into transmission routes. A cross-sectional prevalence study was conducted on 50 randomly selected Dutch broiler farms. Cloacal swabs were taken from 20 randomly chosen broilers. Faecal swabs were returned by 141 individuals living and/or working on 47 farms. ESBL/AmpC-producing E. coli were isolated and, for selected isolates, phylogenetic groups, plasmids and sequence types were determined. Questionnaires were used for risk factor analysis. All sampled farms were positive, with 96.4% positive pooled broiler samples. The human prevalence was 19.1%, with 14.3% and 27.1% among individuals having a low and a high degree of contact with live broilers, respectively. Five pairs of human-broiler isolates had identical genes, plasmid families and E. coli sequence types, showing clonal transmission. Furthermore, similar ESBL/AmpC genes on the same plasmid families in different E. coli sequence types in humans and broilers hinted at horizontal gene transfer. The prevalence among people on broiler farms was higher than in previous studies involving patients and the general population. Furthermore, an increased risk of carriage was shown among individuals having a high degree of contact with live broilers. The (relative) contribution of transmission routes that might play a role in the dissemination of ESBL/AmpC-encoding resistance genes to humans on broiler farms should be pursued in future studies.Keywords
This publication has 20 references indexed in Scilit:
- Identical plasmid AmpC beta-lactamase genes and plasmid types in E. coli isolates from patients and poultry meat in the NetherlandsInternational Journal of Food Microbiology, 2013
- Prevalence of extended-spectrum β-lactamase-producing Enterobacteriaceae in humans living in municipalities with high and low broiler densityClinical Microbiology & Infection, 2013
- Extended-spectrum- -lactamase- and AmpC- -lactamase-producing Escherichia coli in Dutch broilers and broiler farmersJournal of Antimicrobial Chemotherapy, 2012
- Prevalence of livestock-associated MRSA on Dutch broiler farms and in people living and/or working on these farmsEpidemiology and Infection, 2012
- Extended-Spectrum B-Lactamase Genes of Escherichia coli in Chicken Meat and Humans, the NetherlandsEmerging Infectious Diseases, 2011
- In situ ESBL conjugation from avian to human Escherichia coli during cefotaxime administrationJournal of Applied Microbiology, 2010
- Prevalence of livestock-associated MRSA in broiler flocks and risk factors for slaughterhouse personnel in The NetherlandsEpidemiology and Infection, 2010
- Comparative analysis of extended-spectrum- -lactamase-carrying plasmids from different members of Enterobacteriaceae isolated from poultry, pigs and humans: evidence for a shared -lactam resistance gene pool?Journal of Antimicrobial Chemotherapy, 2009
- Diversity of Extended-Spectrum β-Lactamases and Class C β-Lactamases among Cloacal Escherichia coli Isolates in Belgian Broiler FarmsAntimicrobial Agents and Chemotherapy, 2008
- Rapid and Simple Determination of the Escherichia coli Phylogenetic GroupApplied and Environmental Microbiology, 2000