B‐1 cells in the bone marrow are a significant source of natural IgM
Open Access
- 18 October 2011
- journal article
- research article
- Published by Wiley in European Journal of Immunology
- Vol. 42 (1), 120-129
- https://doi.org/10.1002/eji.201141890
Abstract
Natural IgM antibodies secreted in the absence of antigenic challenge are important contributors to antimicrobial immunity and tissue homeostasis. Early studies identified BM and, to a lesser extent the spleen, as main tissue sources of this spontaneously secreted IgM. However, the responsible B‐cell subset has never been identified. Using multicolor flow cytometry, cell sorting and chimeric mice in which B‐1 and B‐2 cells and their secreted antibodies are distinguished by their Ig‐allotype, we unequivocally identify the natural IgM‐secreting cells in spleen and, for the first time, in the BM as IgM+ IgDlo/‐CD19hi CD43+ CD5+/− B‐1 cells. The newly identified population of BM B‐1 cells shows many of the phenotypic characteristics of splenic B‐1 cells but is distinct from B‐1 cells in the peritoneal cavity, which generate at best very small amounts of IgM. Antibody‐secreting spleen and BM B‐1 cells are distinct also from terminally differentiated plasma cells generated from antigen‐induced conventional B cells, as they express high levels of surface IgM and CD19 and lack expression of CD138. Overall, these data identify populations of non‐terminally differentiated B‐1 cells in spleen and BM as the most significant producers of natural IgM.Keywords
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