EWS/FLI1-induced manic fringe renders NIH 3T3 cells tumorigenic
- 1 December 1997
- journal article
- Published by Springer Science and Business Media LLC in Nature Genetics
- Vol. 17 (4), 495-497
- https://doi.org/10.1038/ng1297-495
Abstract
EWS/FLI1, a fusion gene found in Ewing's sarcoma, encodes a transcriptional regulator and promotes cellular transformation by modulating the transcription of specific target genes. We have found that EWS/FLI1 and structurally related fusion proteins upregulate manic fringe (MFNG), a recently described member of the Fringe gene family instrumental in somatic development. MFNG is also expressed in human tumour-derived cell lines expressing EWS/FLI1. Overexpression of MFNG in NIH 3T3 cells renders them tumorigenic in mice with severe combined immunodeficiency disease (SCID). These data demonstrate that part of the oncogenic effect of EWS/FLI1 is to transcriptionally deregulate a member of a family of morphogenic genes.Keywords
This publication has 20 references indexed in Scilit:
- Fringe boundaries coincide with Notch-dependent patterning centres in mammals and alter Notch-dependent development in DrosophilaNature Genetics, 1997
- Secreted Fringe-like Signaling Molecules May Be GlycosyltransferasesCell, 1997
- fringe, a boundary-specific signaling molecule, mediates interactions between dorsal and ventral cells during Drosophila wing developmentCell, 1994
- DNA-binding and transcriptional activation properties of the EWS-FLI-1 fusion protein resulting from the t(11;22) translocation in Ewing sarcoma.Molecular and Cellular Biology, 1994
- A second Ewing's sarcoma translocation, t(21;22), fuses the EWS gene to another ETS–family transcription factor, ERGNature Genetics, 1994
- The Ewing's sarcoma EWS/FLI-1 fusion gene encodes a more potent transcriptional activator and is a more powerful transforming gene than FLI-1.Molecular and Cellular Biology, 1993
- Reverse Transcriptase PCR Amplification of EWS/FLI-1 Fusion Transcripts as a Diagnostic Test for Peripheral Primitive Neuroectodermal Tumors of ChildhoodDiagnostic Molecular Pathology, 1993
- Ewing sarcoma 11;22 translocation produces a chimeric transcription factor that requires the DNA-binding domain encoded by FLI1 for transformation.Proceedings of the National Academy of Sciences of the United States of America, 1993
- Gene fusion with an ETS DNA-binding domain caused by chromosome translocation in human tumoursNature, 1992
- Cytogenetic characterization of selected small round cell tumors of childhoodCancer Genetics and Cytogenetics, 1986