Lipid-Based Passivation in Nanofluidics
Open Access
- 6 April 2012
- journal article
- research article
- Published by American Chemical Society (ACS) in Nano Letters
- Vol. 12 (5), 2260-2265
- https://doi.org/10.1021/nl204535h
Abstract
Stretching DNA in nanochannels is a useful tool for direct, visual studies of genomic DNA at the single molecule level. To facilitate the study of the interaction of linear DNA with proteins in nanochannels, we have implemented a highly effective passivation scheme based on lipid bilayers. We demonstrate virtually complete long-term passivation of nanochannel surfaces to a range of relevant reagents, including streptavidin-coated quantum dots, RecA proteins, and RecA–DNA complexes. We show that the performance of the lipid bilayer is significantly better than that of standard bovine serum albumin-based passivation. Finally, we show how the passivated devices allow us to monitor single DNA cleavage events during enzymatic degradation by DNase I. We expect that our approach will open up for detailed, systematic studies of a wide range of protein–DNA interactions with high spatial and temporal resolution.Keywords
This publication has 24 references indexed in Scilit:
- Single-molecule denaturation mapping of DNA in nanofluidic channelsProceedings of the National Academy of Sciences, 2010
- Mechanical Behavior of a Supported Lipid Bilayer under External Shear ForcesLangmuir, 2009
- Local Conformation of Confined DNA Studied Using Emission Polarization AnisotropySmall, 2009
- Cutting the forest to see a single tree?Nature Chemical Biology, 2008
- Long-distance lateral diffusion of human Rad51 on double-stranded DNAProceedings of the National Academy of Sciences, 2006
- An Aqueous-Based Surface Modification of Poly(dimethylsiloxane) with Poly(ethylene glycol) to Prevent BiofoulingLangmuir, 2005
- Wetting of phospholipid membranes on hydrophilic surfaces - Concepts towards self-healing membranesZeitschrift für Physik B Condensed Matter, 1999
- Phenomenology and Kinetics of Lipid Bilayer Spreading on Hydrophilic SurfacesLangmuir, 1995
- CRYSTALLINE DESOXYRIBONUCLEASEThe Journal of general physiology, 1950
- CRYSTALLINE DESOXYRIBONUCLEASEThe Journal of general physiology, 1950