The Fate of the Adipose-Derived Stromal Cells during Angiogenesis and Adipogenesis after Cell-Assisted Lipotransfer
- 1 February 2018
- journal article
- research article
- Published by Ovid Technologies (Wolters Kluwer Health) in Plastic and Reconstructive Surgery
- Vol. 141 (2), 365-375
- https://doi.org/10.1097/prs.0000000000004021
Abstract
Background: Cell-assisted lipotransfer is a process in which fat grafting is supplemented with autologous adipose-derived stromal cells. Since the efficacy of the technique was demonstrated, studies have focused on the mechanism by which cell-assisted lipotransfer enhances the rate of graft survival. However, the microenvironmental changes in donor and recipient tissue associated with cell-assisted lipotransfer remain unclear. Methods: The authors introduced an animal model of cell-assisted lipotransfer using two different transgenic reporter mice. Donor fat from green fluorescent protein–expressing C57BL/6J mice and donor adipose-derived stromal cells from DsRed-expressing C57BL/6J mice were co-transplanted into recipient C57BL/6J mice. During adipose remodeling after cell-assisted lipotransfer, the fate of each donor adipocyte and donor adipose-derived stromal cell was traced using immunofluorescent staining with the whole-mount method. Results: Adipose-derived stromal cell supplementation altered inflammation and promoted angiogenesis and subsequent revascularization in recipient tissue. Tracing at postoperative week 4 revealed that surviving donor adipose-derived stromal cells participated in angiogenesis by differentiating into endothelial cells. Moreover, newly differentiated fat from donor adipose-derived stromal cells and recipient tissue integrated with surviving donor fat, leading to improved retention of the graft. Adipose-derived stromal cell supplementation resulted in a quantitative difference in angiogenesis and adipogenesis during adipose remodeling according to the concentration of adipose-derived stromal cells. Conclusions: The authors characterized the dynamic changes occurring in donor adipose-derived stromal cells and fat and recipient tissue by tracing these cellular components following cell-assisted lipotransfer. The authors’ findings highlight the therapeutic value of cell-assisted lipotransfer in tissue transplantation.Keywords
This publication has 34 references indexed in Scilit:
- Cell-Assisted Lipotransfer for the Treatment of Parry-Romberg SyndromeArchives of Plastic Surgery, 2012
- The Fate of Adipocytes after Nonvascularized Fat GraftingPlastic and Reconstructive Surgery, 2012
- Lineage TracingCell, 2012
- The spatiotemporal development of adipose tissueDevelopment, 2011
- Quantification of adiposity in small rodents using micro-CTMethods, 2010
- Angiogenesis modulates adipogenesis and obesityJCI Insight, 2007
- Cell-Assisted Lipotransfer for Cosmetic Breast Augmentation: Supportive Use of Adipose-Derived Stem/Stromal CellsAesthetic Plastic Surgery, 2007
- Angiogenic Role of LYVE-1–Positive Macrophages in Adipose TissueCirculation Research, 2007
- Cell-Assisted Lipotransfer: Supportive Use of Human Adipose-Derived Cells for Soft Tissue Augmentation with LipoinjectionTissue Engineering, 2006
- Plasticity of Human Adipose Lineage Cells Toward Endothelial CellsCirculation, 2004