Rapid and efficient detection of genetic polymorphism in wheat through amplification by polymerase chain reaction
- 1 July 1990
- journal article
- research article
- Published by Springer Science and Business Media LLC in Plant Molecular Biology
- Vol. 15 (1), 169-171
- https://doi.org/10.1007/bf00017737
Abstract
The polymerase chain reaction (PCR) was used to amplify genomic DNA of several wheat genotypes. The oligonucleotides used as primers were the terminal sequences of a gamma-gliadin gene. The electrophoretic analysis of the PCR products showed specific bands which revealed both inter- and intra-specific genetic polymorphism among the examined genotypes. The technique is proposed as a very simple and efficient alternative to RFLP markers.This publication has 5 references indexed in Scilit:
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