Dopamine D1/D5 Receptor Modulates State-Dependent Switching of Soma-Dendritic Ca2+Potentials via Differential Protein Kinase A and C Activation in Rat Prefrontal Cortical Neurons
Open Access
- 7 January 2004
- journal article
- Published by Society for Neuroscience in Journal of Neuroscience
- Vol. 24 (1), 8-23
- https://doi.org/10.1523/jneurosci.1650-03.2004
Abstract
To determine the nature of dopamine modulation of dendritic Ca2+signaling in layers V-VI prefrontal cortex (PFC) neurons, whole-cell Ca2+potentials were evoked after blockade of Na+and K+channels. Soma-dendritic Ca2+spikes evoked by suprathreshold depolarizing pulses, which could be terminated by superimposed brief intrasomatic hyperpolarizing pulses, are blocked by the L-type Ca2+channel antagonist nimodipine (1 μm). The D1/D5 receptor agonist dihydrexidine (DHX) (0.01-10 μm; 5 min) or R-(+)SKF81291 (10 μm) induced a prolonged (>30 min) dose-dependent peak suppression of these Ca2+spikes. This effect was dependent on [Ca2+]i- and protein kinase C (PKC)-dependent mechanisms because [Ca2+]ichelation by BAPTA or inhibition of PKC by bisindolymaleimide (BiM1), but not inhibition of [Ca2+]irelease with heparin or Xestospongin C, prevented the D1-mediated suppression of Ca2+spikes. Depolarizing pulses subthreshold to activating a Ca2+spike evoked a nimodipine-sensitive Ca2+“hump” potential. D1/D5 stimulation induced anN-[2-((o-bromocinamyl)amino)ethyl]-5-isoquinolinesulfonamide (H-89)- or internal PKA inhibitory peptide[5-24]-sensitive (PKA-dependent) transient (∼7 min) potentiation of the hump potential to full Ca2+spike firing. Furthermore, application of DHX in the presence of the PKC inhibitor BiM1 or internal PKC inhibitory peptide[19-36]resulted in persistent firing of full Ca2+spike bursts, suggesting that a D1/D5-PKA mechanism switches subthreshold Ca2+hump potential to fire full Ca2+spikes, which are eventually turned off by a D1/D5-Ca2+-dependent PKC mechanism. This depolarizing state-dependent, D1/D5-activated, bi-directional switching of soma-dendritic L-type Ca2+channels via PKA-dependent potentiation and PKC-dependent suppression may provide spatiotemporal regulation of synaptic integration and plasticity in PFC.This publication has 92 references indexed in Scilit:
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