Identification of Two Porcine Reproductive and Respiratory Syndrome Virus Variants Sharing High Genomic Homology but with Distinct Virulence
Open Access
- 18 September 2019
- Vol. 11 (9), 875
- https://doi.org/10.3390/v11090875
Abstract
Porcine reproductive and respiratory syndrome virus (PRRSV) causes huge economic loss to the global swine industry. Even though several control strategies have been applied, PRRS is still not effectively controlled due to the continuous emergence of new variants and limited cross-protection by current vaccines. During the routine epidemiological investigation in 2017, two PRRSV variants were identified from a severe abortion farm and a clinically healthy farm, respectively. The viruses were isolated and denominated as XJ17-5 and JSTZ1712-12. Genomic sequencing indicated that their genomes are both 14,960 bp in length sharing 99.45% nucleotide identity. Sequence alignments identified a discontinuous 30-amino-acid deletion and a continuous 120-amino-acid deletion in nsp2 of both isolates. Genome-based phylogenetic analysis confirmed that XJ17-5 and JSTZ1712-12 belong to the HP-PRRSV subtype but form a new branch with other isolates containing the same 150-amino-acid deletion in nsp2. Pathogenic analysis showed that XJ17-5 is highly virulent causing 60% mortality, while JSTZ1712-12 is avirulent for piglets. Furthermore, fragment comparisons identified 34-amino-acid differences between XJ17-5 and JSTZ1712-12 that might be associated with the distinct virulence. The identification of highly homologous HP-PRRSV variants with new genetic feature and distinct virulence contributes to further analyze the pathogenesis and evolution of PRRSV in the field.Keywords
Funding Information
- National Natural Science Foundation of China (31802172)
- Natural Science Foundation of Jiangsu Province (BK20170492)
- China Postdoctoral Science Foundation (2016M590510)
This publication has 55 references indexed in Scilit:
- Evaluation of viral peptide targeting to porcine sialoadhesin using a porcine reproductive and respiratory syndrome virus vaccination-challenge modelVirus Research, 2013
- Efficient −2 frameshifting by mammalian ribosomes to synthesize an additional arterivirus proteinProceedings of the National Academy of Sciences of the United States of America, 2012
- Mutations in the genome of the highly pathogenic porcine reproductive and respiratory syndrome virus potentially related to attenuationVeterinary Microbiology, 2012
- Nonstructural Protein 2 of Porcine Reproductive and Respiratory Syndrome Virus Inhibits the Antiviral Function of Interferon-Stimulated Gene 15Journal of Virology, 2012
- Recombination in Vaccine and Circulating Strains of Porcine Reproductive and Respiratory Syndrome VirusesEmerging Infectious Diseases, 2009
- The 30-Amino-Acid Deletion in the Nsp2 of Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus Emerging in China Is Not Related to Its VirulenceJournal of Virology, 2009
- Different Biological Characteristics of Wild-Type Porcine Reproductive and Respiratory Syndrome Viruses and Vaccine Viruses and Identification of the Corresponding Genetic DeterminantsJournal of Clinical Microbiology, 2008
- Clustal W and Clustal X version 2.0Bioinformatics, 2007
- Highly Pathogenic Porcine Reproductive and Respiratory Syndrome, ChinaEmerging Infectious Diseases, 2007
- Emergence of Fatal PRRSV Variants: Unparalleled Outbreaks of Atypical PRRS in China and Molecular Dissection of the Unique HallmarkPLOS ONE, 2007