Supplementary Methods, Figures S1 - S10, Tables S1 - S2 from HOXB7 Is an ERα Cofactor in the Activation of HER2 and Multiple ER Target Genes Leading to Endocrine Resistance

Abstract

Supplementary Figure S1 (related to Figure 1). HOXB7 microarray and ChIP-PED analysis. Supplementary Figure S2 (related to Figure 1). HOXB7 enhances expression of ER target genes. Supplementary Figure S3 (related to Figure 2). Validation of HOXB7 binding events to ER target genes. Supplementary Figure S4 (related to Figure 2). HOXB7 is required for ER target gene expression. Supplementary Figure S5 (related to Figure 3). Correlation between HOXB7 and HER2 expression. Supplementary Figure S6 (related to Figure 3). HOXB7 regulates HER2 expression. Supplementary Figure S7 (related to Figure 4). miRNA-196a regulates HOXB7 expression. Supplementary Figure S8 (related to Figure 5). MYC and miRNA-196a regulates HOXB7 expression. Supplementary Figure S9 (related to Figure 5). In vivo analysis of effects of inhibiting MYC and HER2 to overcome TAM-resistance in BT474 cells. Supplementary Figure S10 (related to Figure 6). MYC-HOXB7-HER2 predicts clinical outcome of breast cancer in women treated with endocrine therapy. Supplementary Table S1 (related to Figure 1), Family wise error rate (FWER) for MR-GSE of genes differentially expressed between MCF-7-HOXB7 and MCF-7-Vector cells. Supplementary Table S2 (related to Figure 1). ER target genes ranked in order of the average correlation with HOXB7 or ER.