Reduced Hippocampal Dendritic Spine Density and BDNF Expression following Acute Postnatal Exposure to Di(2-Ethylhexyl) Phthalate in Male Long Evans Rats
Open Access
- 8 October 2014
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 9 (10), e109522
- https://doi.org/10.1371/journal.pone.0109522
Abstract
Early developmental exposure to di(2-ethylhexyl) phthalate (DEHP) has been linked to a variety of neurodevelopmental changes, particularly in rodents. The primary goal of this work was to establish whether acute postnatal exposure to a low dose of DEHP would alter hippocampal dendritic morphology and BDNF and caspase-3 mRNA expression in male and female Long Evans rats. Treatment with DEHP in male rats led to a reduction in spine density on basal and apical dendrites of neurons in the CA3 dorsal hippocampal region compared to vehicle-treated male controls. Dorsal hippocampal BDNF mRNA expression was also down-regulated in male rats exposed to DEHP. No differences in hippocampal spine density or BDNF mRNA expression were observed in female rats treated with DEHP compared to controls. DEHP treatment did not affect hippocampal caspase-3 mRNA expression in male or female rats. These results suggest a gender-specific vulnerability to early developmental DEHP exposure in male rats whereby postnatal DEHP exposure may interfere with normal synaptogenesis and connectivity in the hippocampus. Decreased expression of BDNF mRNA may represent a molecular mechanism underlying the reduction in dendritic spine density observed in hippocampal CA3 neurons. These findings provide initial evidence for a link between developmental exposure to DEHP, reduced levels of BDNF and hippocampal atrophy in male rats.Keywords
This publication has 63 references indexed in Scilit:
- Activation of Trim17 by PPARγ is involved in Di(2-ethylhexyl) phthalate (DEHP)-induced apoptosis on Neuro-2a cellsToxicology Letters, 2011
- Acute postnatal exposure to di(2-ethylhexyl) phthalate adversely impacts hippocampal development in the male ratNeuroscience, 2011
- Reproductive and Developmental Toxicity of PhthalatesJournal of Toxicology and Environmental Health, Part B, 2009
- Analyzing real-time PCR data by the comparative CT methodNature Protocols, 2008
- Biphasic Effects of Postnatal Exposure to Diethylhexylphthalate on the Timing of Puberty in Male RatsJournal of Andrology, 2007
- Di(2‐ethylhexyl)phthalate (DEHP): human metabolism and internal exposure – an update and latest results1International Journal of Andrology, 2006
- Estrogen induces rapid decrease in dendritic thorns of CA3 pyramidal neurons in adult male rat hippocampusBiochemical and Biophysical Research Communications, 2005
- Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT MethodMethods, 2001
- Testosterone attenuates β-amyloid toxicity in cultured hippocampal neuronsBrain Research, 2001
- Participation of the Fas-Signaling System in the Initiation of Germ Cell Apoptosis in Young Rat Testes after Exposure to Mono-(2-Ethylhexyl) PhthalateToxicology and Applied Pharmacology, 1999