Offspring from Oocytes Derived from in Vitro Primordial Germ Cell–like Cells in Mice
Top Cited Papers
- 16 November 2012
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 338 (6109), 971-975
- https://doi.org/10.1126/science.1226889
Abstract
Reconstitution of female germ cell development in vitro is a key challenge in reproductive biology and medicine. We show here that female (XX) embryonic stem cells and induced pluripotent stem cells in mice are induced into primordial germ cell–like cells (PGCLCs), which, when aggregated with female gonadal somatic cells as reconstituted ovaries, undergo X-reactivation, imprint erasure, and cyst formation, and exhibit meiotic potential. Upon transplantation under mouse ovarian bursa, PGCLCs in the reconstituted ovaries mature into germinal vesicle-stage oocytes, which then contribute to fertile offspring after in vitro maturation and fertilization. Our culture system serves as a robust foundation for the investigation of key properties of female germ cells, including the acquisition of totipotency, and for the reconstitution of whole female germ cell development in vitro.Keywords
This publication has 37 references indexed in Scilit:
- Reconstitution of the Mouse Germ Cell Specification Pathway in Culture by Pluripotent Stem CellsCell, 2011
- Nanog Is the Gateway to the Pluripotent Ground StateCell, 2009
- QUMA: quantification tool for methylation analysisNucleic Acids Research, 2008
- Of lineage and legacy: the development of mammalian hematopoietic stem cellsNature Immunology, 2008
- [9] TM4 Microarray Software SuiteMethods in Enzymology, 2006
- Dynamic chromatin modifications characterise the first cell cycle in mouse embryosDevelopmental Biology, 2005
- Extensive and orderly reprogramming of genome-wide chromatin modifications associated with specification and early development of germ cells in miceDevelopmental Biology, 2005
- Derivation of Oocytes from Mouse Embryonic Stem CellsScience, 2003
- Development in vitro of mouse oocytes from primordial folliclesBiology of Reproduction, 1996
- Mouse Offspring Derived from Fetal Ovaries or Reaggregates Which were Cultured and Transplanted into Adult FemalesDevelopment, Growth & Differentiation, 1992