Betaine Improves the PCR Amplification of GC-Rich DNA Sequences
Open Access
- 1 October 1997
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 25 (19), 3957-3958
- https://doi.org/10.1093/nar/25.19.3957
Abstract
Betaine improves the co-amplification of the two alternatively spliced variants of the prostate-specific membrane antigen mRNA as well as the amplification of the coding cDNA region of c-jun. It is suggested that betaine improves the amplification of these genes by reducing the formation of secondary structure caused by GC-rich regions and, therefore, may be generally applicable to ameliorate the amplification of GC-rich DNA sequences.Keywords
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