A protocol for isolation and culture of human umbilical vein endothelial cells
- 15 March 2007
- journal article
- Published by Springer Science and Business Media LLC in Nature Protocols
- Vol. 2 (3), 481-485
- https://doi.org/10.1038/nprot.2007.54
Abstract
We describe a protocol for easy isolation and culture of human umbilical vein endothelial cells (HUVECs) to supply every researcher with a method that can be applied in cell biology laboratories with minimum equipment. Endothelial cells (ECs) are isolated from umbilical vein vascular wall by a collagenase treatment, then seeded on fibronectin-coated plates and cultured in a medium with Earles' salts and fetal calf serum (FCS), but without growth factor supplementation, for 7 days in a 37 degrees C-5% CO2 incubator. Cell confluency can be monitored by phase-contrast microscopy; ECs can be characterized using cell surface or intracellular markers and checked for contamination. Various protocols can be applied to HUVECs, from simple harvesting to a particular solubilization of proteins for proteomic analysis.Keywords
This publication has 28 references indexed in Scilit:
- PHProteomicDB: A Module for Two-dimensional Gel Electrophoresis Database Creation on Personal Web SitesGenomics, Proteomics and Bioinformatics, 2006
- Proteomics of human umbilical vein endothelial cells applied to etoposide‐induced apoptosisProteomics, 2005
- Cyclosporin A But Not Estradiol Can Protect Endothelial Cells Against Etoposide-Induced ApoptosisEndothelium, 2004
- Captopril and enalaprilat decrease antioxidant defences in human endothelial cells and are unable to protect against apoptosisCell Biology International, 2003
- Proteomic study of human umbilical vein endothelial cells in cultureProteomics, 2003
- Anticancer drugs induce necrosis of human endothelial cells involving both oncosis and apoptosisEuropean Journal of Cell Biology, 2001
- Regulatory Functions of the Vascular EndotheliumThe New England Journal of Medicine, 1990
- Endothelial CellsArchives of Internal Medicine, 1987
- Endothelial injury caused by antineoplastic agentsBiochemical Pharmacology, 1986
- Editorial boardExperimental pathology, 1984