Comparison of p16INK4a and ProEx™ C immunostaining on cervical ThinPrep® cytology and biopsy specimens
- 20 July 2010
- journal article
- research article
- Published by Wiley in Diagnostic Cytopathology
- Vol. 38 (8), 564-572
- https://doi.org/10.1002/dc.21251
Abstract
ProEx™ C and p16INK4a staining of cytology/histology specimens have recently been explored to help distinguish high‐grade squamous intraepithelial lesions (HSIL) from benign mimics. The goal of this study was to evaluate the performance characteristics of p16 and ProEx C in tissue and patient matched ThinPrep® liquid‐based cytology specimens. Residual cervical ThinPrep cytology specimens and tissue blocks (N = 64) from 63 patients were stained with p16 and ProEx C. Review of immunostained material, Papanicolaou and H&E stained slides was performed by two cytopathologists. The cytology slides were evaluated for the presence or absence of squamous atypia as well as immunoreactivity. Histologic specimens were interpreted as negative, indeterminate, or positive for each immunostain. There was 86% agreement (55/64) between the p16 and ProEx C stains on tissue specimens. Eleven specimens were interpreted as positive for both stains. All had a low‐ or high‐grade squamous lesion on the corresponding H&E section. ProEx C was able to identify four low‐grade squamous intraepithelial lesion specimens that were interpreted as negative by p16. All four HSIL specimens demonstrated p16 and ProEx C staining. However, 84% of cytology negative specimens demonstrated false‐positive staining. Clinical utilization of both stains, combined with morphologic features, may be beneficial for confirming HSIL on histologic specimens. ProEx C and/or p16 immunostains may lead to a false‐positive result in cytology specimens due to staining of normal appearing cells. Diagn. Cytopathol. 2010;38:564–572. 2009 Wiley‐Liss, Inc.Keywords
This publication has 26 references indexed in Scilit:
- Comparison of ProEx C with p16INK4a and Ki-67 immunohistochemical staining of cell blocks prepared from residual liquid-based cervicovaginal materialCancer, 2008
- Detecting High-Grade Cervical Disease on ASC-H CytologyAmerican Journal of Clinical Pathology, 2008
- Clinical Role of p16INK4aExpression in Liquid-Based Cervical CytologyAmerican Journal of Clinical Pathology, 2008
- Evaluation of p16INK4a as a diagnostic tool in the triage of Pap smears demonstrating atypical squamous cells of undetermined significanceCancer, 2008
- Evaluation of p16INK4a expression in ThinPrep cervical specimens with the CINtec p16INK4a assayCancer, 2007
- A comparison of the clinical utility of p16INK4a immunolocalization with the presence of human papillomavirus by hybrid capture 2 for the detection of cervical dysplasia/neoplasiaCancer, 2006
- Trichomonas vaginalis P16 Immunoreactivity in cervicovaginal Pap tests: A diagnostic pitfallDiagnostic Cytopathology, 2005
- Immunocytochemical staining of p16INK4a protein from conventional Pap test and its association with human papillomavirus infectionDiagnostic Cytopathology, 2004
- The utility of p16INK4a and Ki-67 staining on cell blocks prepared from residual thin-layer cervicovaginal materialCancer, 2004
- Overexpression of p16INK4A in liquid‐based specimens (SurePath™) as marker of cervical dysplasia and neoplasiaDiagnostic Cytopathology, 2002