A review of chromatographic methods for the determination of water‐ and fat‐soluble vitamins in biological fluids
- 25 November 2015
- journal article
- review article
- Published by Wiley in Journal of Separation Science
- Vol. 39 (1), 132-148
- https://doi.org/10.1002/jssc.201501038
Abstract
Vitamins are an essential element of nutrition and thus contribute to human health. Vitamins catalyze many biochemical reactions and their lack or excess can cause health problems. Therefore, monitoring vitamin concentrations in plasma or other biological fluids may be useful in the diagnosis of various disorders as well as in the treatment process. Several chromatographic methods have been developed for the determination of these compounds in biological samples, including high-performance liquid chromatography with UV and fluorescence detection. Recently, high-performance liquid chromatography with tandem mass spectrometry methods have been widely used for the determination of vitamins in complex matrices because of their high sensitivity and selectivity. This method requires preconditioning of samples for analysis, including protein precipitation and/or various extraction techniques. The choice of method may depend on the desired cost, convenience, turnaround time, specificity, and accuracy of the information to be obtained. This article reviews the recently reported chromatographic methods used for determination of vitamins in biological fluids. Relevant papers published mostly during the last 5 years were identified by an extensive PubMed search using appropriate keywords. Particular attention was given to the preparation steps and extraction techniques. This report may be helpful in the selection of procedures that are appropriate for certain types of biological materials and analytes.This publication has 77 references indexed in Scilit:
- Comparison of high‐performance liquid chromatography/tandem mass spectrometry and high‐performance liquid chromatography/photo‐diode array detection for the quantitation of carotenoids, retinyl esters, α‐tocopherol and phylloquinone in chylomicron‐rich fractions of human plasmaRapid Communications in Mass Spectrometry, 2013
- An LC/MS method for d8-β-carotene and d4-retinyl esters: β-carotene absorption and its conversion to vitamin A in humansJournal of Lipid Research, 2012
- A sensitive and specific method for measurement of multiple retinoids in human serum with UHPLC-MS/MSJournal of Lipid Research, 2012
- Determination of lipoic acid, Trolox methyl ether and tocopherols in human plasma by liquid‐chromatography and ion‐trap tandem mass spectrometryBiomedical Chromatography, 2012
- A Novel HPLC Method for the Concurrent Analysis and Quantitation of Seven Water-Soluble Vitamins in Biological Fluids (Plasma and Urine): A Validation Study and ApplicationThe Scientific World Journal, 2012
- Effects of niacin on atherosclerosis and vascular functionCurrent Opinion in Cardiology, 2011
- Vitamin D Deficiency and Risk of Cardiovascular DiseaseCirculation, 2008
- Identification and determination of fat‐soluble vitamins and metabolites in human serum by liquid chromatography/triple quadrupole mass spectrometry with multiple reaction monitoringRapid Communications in Mass Spectrometry, 2007
- Vitamin K: The coagulation vitamin that became omnipotentThrombosis and Haemostasis, 2007
- Mitochondrial function and toxicity: Role of the B vitamin family on mitochondrial energy metabolismChemico-Biological Interactions, 2006