Bromodomain-dependent stage-specific male genome programming by Brdt
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Open Access
- 24 August 2012
- journal article
- research article
- Published by Springer Science and Business Media LLC in The EMBO Journal
- Vol. 31 (19), 3809-3820
- https://doi.org/10.1038/emboj.2012.233
Abstract
Male germ cell differentiation is a highly regulated multistep process initiated by the commitment of progenitor cells into meiosis and characterized by major chromatin reorganizations in haploid spermatids. We report here that a single member of the double bromodomain BET factors, Brdt, is a master regulator of both meiotic divisions and post‐meiotic genome repackaging. Upon its activation at the onset of meiosis, Brdt drives and determines the developmental timing of a testis‐specific gene expression program. In meiotic and post‐meiotic cells, Brdt initiates a genuine histone acetylation‐guided programming of the genome by activating essential genes and repressing a ‘progenitor cells’ gene expression program. At post‐meiotic stages, a global chromatin hyperacetylation gives the signal for Brdt's first bromodomain to direct the genome‐wide replacement of histones by transition proteins. Brdt is therefore a unique and essential regulator of male germ cell differentiation, which, by using various domains in a developmentally controlled manner, first drives a specific spermatogenic gene expression program, and later controls the tight packaging of the male genome. There is a [Have you seen?][1] (October 2012) associated with this Article. [1]: http://dx.doi.org/10.1038/emboj.2012.259Keywords
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