Effect of Honey on Streptococcus mutans Growth and Biofilm Formation
Open Access
- 15 January 2012
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 78 (2), 536-540
- https://doi.org/10.1128/aem.05538-11
Abstract
Because of the tradition of using honey as an antimicrobial medicament, we investigated the effect of natural honey (NH) on Streptococcus mutans growth, viability, and biofilm formation compared to that of an artificial honey (AH). AH contained the sugars at the concentrations reported for NH. NH and AH concentrations were obtained by serial dilution with tryptic soy broth (TSB). Several concentrations of NH and AH were tested for inhibition of bacterial growth, viability, and biofilm formation after inoculation with S. mutans UA159 in 96-well microtiter plates to obtain absorbance and CFU values. Overall, NH supported significantly less ( P < 0.05) bacterial growth than AH at 25 and 12.5% concentrations. At 50 and 25% concentrations, both honey groups provided significantly less bacterial growth and biofilm formation than the TSB control. For bacterial viability, the results for all honey concentrations except 50% NH were not significantly different from those for the TSB control. NH was able to decrease the maximum velocity of S. mutans growth compared to AH. In summary, NH demonstrated more inhibition of bacterial growth, viability, and biofilm formation than AH. This study highlights the potential antibacterial properties of NH and could suggest that the antimicrobial mechanism of NH is not solely due to its high sugar content.Keywords
This publication has 41 references indexed in Scilit:
- Two Major Medicinal Honeys Have Different Mechanisms of Bactericidal ActivityPLOS ONE, 2011
- How honey kills bacteriaThe FASEB Journal, 2010
- Standardized antibacterial honey (Medihoney™) with standard therapy in wound care: randomized clinical trialJournal of Advanced Nursing, 2009
- Biologic Dressing in BurnsThe Journal of Craniofacial Surgery, 2008
- The Role of Chlorhexidine in Caries PreventionOperative Dentistry, 2008
- Antibacterial Activity of 13 Honeys Against Escherichia coli and Pseudomonas aeruginosaJournal of Medicinal Food, 2005
- A comparison of the sensitivity of wound‐infecting species of bacteria to the antibacterial activity of manuka honey and other honeyJournal of Applied Bacteriology, 1992
- A Survey of the Antibacterial Activity of Some New Zealand HoneysJournal of Pharmacy and Pharmacology, 1991
- Turku sugar studies V: Final report on the effect of sucrose, fructose and xylitol diets on the caries incidence in manActa Odontologica Scandinavica, 1976
- The identification of inhibine, the antibacterial factor in honey, as hydrogen peroxide and its origin in a honey glucose-oxidase systemBiochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects, 1963