Temporal Analysis of Active and Passive Transport in Living Cells
- 12 December 2008
- journal article
- research article
- Published by American Physical Society (APS) in Physical Review Letters
- Vol. 101 (24), 248103
- https://doi.org/10.1103/physrevlett.101.248103
Abstract
The cellular cytoskeleton is a fascinating active network, in which Brownian motion is intercepted by distinct phases of active transport. We present a time-resolved statistical analysis dissecting phases of directed motion out of otherwise diffusive motion of tracer particles in living cells. The distribution of active lifetimes is found to decay exponentially with a characteristic time . The velocity distribution of active events exhibits several peaks, in agreement with a discrete number of motor proteins acting collectively.
Keywords
This publication has 26 references indexed in Scilit:
- Tug-of-war as a cooperative mechanism for bidirectional cargo transport by molecular motorsProceedings of the National Academy of Sciences of the United States of America, 2008
- Enhanced reaction kinetics in biological cellsNature Physics, 2008
- Directional memory and caged dynamics in cytoskeletal remodellingBiochemical and Biophysical Research Communications, 2007
- Stress fluctuations and motion of cytoskeletal-bound markersPhysical Review E, 2007
- Asymmetric redistribution of GABA receptors during GABA gradient sensing by nerve growth cones analyzed by single quantum dot imagingProceedings of the National Academy of Sciences of the United States of America, 2007
- Active mechanical stabilization of the viscoplastic intracellular space of Dictyostelia cells by microtubule–actin crosstalkActa Biomaterialia, 2006
- The Role of F-Actin and Myosin in Epithelial Cell RheologyBiophysical Journal, 2006
- Analysis of Transient Behavior in Complex Trajectories: Application to Secretory Vesicle DynamicsBiophysical Journal, 2006
- Active Diffusion of Motor ParticlesPhysical Review Letters, 2005
- Visualization of vesicle transport along and between distinct pathways in neurites of living cellsMicroscopy Research and Technique, 2004