Mannose-Binding Lectin in Severe Acute Respiratory Syndrome Coronavirus Infection
Open Access
- 15 May 2005
- journal article
- Published by Oxford University Press (OUP) in The Journal of Infectious Diseases
- Vol. 191 (10), 1697-1704
- https://doi.org/10.1086/429631
Abstract
Little is known about the innate immune response to severe acute respiratory syndrome (SARS) coronavirus (CoV) infection. Mannose-binding lectin (MBL), a key molecule in innate immunity, functions as an ante-antibody before the specific antibody response. Here, we describe a case-control study that included 569 patients with SARS and 1188 control subjects and used in vitro assays to investigate the role that MBL plays in SARS-CoV infection. The distribution of MBL gene polymorphisms was significantly different between patients with SARS and control subjects, with a higher frequency of haplotypes associated with low or deficient serum levels of MBL in patients with SARS than in control subjects. Serum levels of MBL were also significantly lower in patients with SARS than in control subjects. There was, however, no association between MBL genotypes, which are associated with low or deficient serum levels of MBL, and mortality related to SARS. MBL could bind SARS-CoV in a dose- and calcium-dependent and mannan-inhibitable fashion in vitro, suggesting that binding is through the carbohydrate recognition domains of MBL. Furthermore, deposition of complement C4 on SARS-CoV was enhanced by MBL. Inhibition of the infectivity of SARS-CoV by MBL in fetal rhesus kidney cells (FRhK-4) was also observed. These results suggest that MBL contributes to the first-line host defense against SARS-CoV and that MBL deficiency is a susceptibility factor for acquisition of SARSKeywords
This publication has 44 references indexed in Scilit:
- Associations betweenIL12BPolymorphisms and Tuberculosis in the Hong Kong Chinese PopulationThe Journal of Infectious Diseases, 2004
- Serum Mannose-Binding Lectin Levels and mbl2 Gene Polymorphisms in Different Age and Gender Groups of Southern Chinese AdultsScandinavian Journal of Immunology, 2004
- Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirusNature, 2003
- The Genome Sequence of the SARS-Associated CoronavirusScience, 2003
- Characterization of a Novel Coronavirus Associated with Severe Acute Respiratory SyndromeScience, 2003
- Mannose-binding lectin gene polymorphism predicts hospital admissions for COPD infectionsGenes & Immunity, 2003
- Mass Spectrometric Characterization of Proteins from the SARS VirusMolecular & Cellular Proteomics, 2003
- Increased frequency of mannose-binding lectin insufficiency among children with acute lymphoblastic leukemiaBlood, 2002
- Association of mannose-binding lectin gene heterogeneity with severity of lung disease and survival in cystic fibrosisJCI Insight, 1999
- The human mannose-binding protein functions as an opsonin.The Journal of Experimental Medicine, 1989