Multi-layer Born multiple-scattering model for 3D phase microscopy
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- 28 April 2020
- journal article
- research article
- Published by Optica Publishing Group in Optica
- Vol. 7 (5), 394-403
- https://doi.org/10.1364/optica.383030
Abstract
We propose an accurate and computationally efficient 3D scattering model, multi-layer Born (MLB), and use it to recover the 3D refractive index (RI) of thick biological samples. For inverse problems recovering the complex field of thick samples, weak scattering models (e.g., first Born) may fail or underestimate the RI, especially with a large index contrast. Multi-slice (MS) beam propagation methods model multiple scattering to provide more realistic reconstructions; however, MS does not properly account for highly oblique scattering, nor does it model backward scattering. Our proposed MLB model uses a first Born model at each of many slices, accurately capturing the oblique scattering effects and estimating the backward scattering process. When used in conjunction with an inverse solver, the model provides more accurate RI reconstructions for high-resolution phase tomography. Importantly, MLB retains a reasonable computation time that is critical for practical implementation with iterative inverse algorithms.Keywords
Funding Information
- National Science Foundation (DMR 1548924)
- Gordon and Betty Moore Foundation (GBMF4562)
- Office of Naval Research (N00014-17-1-2401)
- David and Lucile Packard Foundation
- Chan Zuckerberg Initiative
- Kirschstein National Research Service Award (F32GM129966)
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