Implication of Bemisia tabaci Heat Shock Protein 70 in Begomovirus-Whitefly Interactions
Open Access
- 15 December 2012
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 86 (24), 13241-13252
- https://doi.org/10.1128/jvi.00880-12
Abstract
The whitefly Bemisia tabaci (Gennadius) is a major cosmopolitan pest capable of feeding on hundreds of plant species and transmits several major plant viruses. The most important and widespread viruses vectored by B. tabaci are in the genus Begomovirus , an unusual group of plant viruses owing to their small, single-stranded DNA genome and geminate particle morphology. B. tabaci transmits begomoviruses in a persistent circulative nonpropagative manner. Evidence suggests that the whitefly vector encounters deleterious effects following Tomato yellow leaf curl virus (TYLCV) ingestion and retention. However, little is known about the molecular and cellular basis underlying these coevolved begomovirus-whitefly interactions. To elucidate these interactions, we undertook a study using B. tabaci microarrays to specifically describe the responses of the transcriptomes of whole insects and dissected midguts following TYLCV acquisition and retention. Microarray, real-time PCR, and Western blot analyses indicated that B. tabaci heat shock protein 70 (HSP70) specifically responded to the presence of the monopartite TYLCV and the bipartite Squash leaf curl virus . Immunocapture PCR, protein coimmunoprecipitation, and virus overlay protein binding assays showed in vitro interaction between TYLCV and HSP70. Fluorescence in situ hybridization and immunolocalization showed colocalization of TYLCV and the bipartite Watermelon chlorotic stunt virus virions and HSP70 within midgut epithelial cells. Finally, membrane feeding of whiteflies with anti-HSP70 antibodies and TYLCV virions showed an increase in TYLCV transmission, suggesting an inhibitory role for HSP70 in virus transmission, a role that might be related to protection against begomoviruses while translocating in the whitefly.Keywords
This publication has 72 references indexed in Scilit:
- Global Analysis of the Transcriptional Response of Whitefly to Tomato Yellow Leaf Curl China Virus Reveals the Relationship of Coevolved AdaptationsJournal of Virology, 2011
- An important role of the heat shock response in infected cells for replication of baculovirusesVirology, 2010
- The Transmission Efficiency of Tomato Yellow Leaf Curl Virus by the Whitefly Bemisia tabaci Is Correlated with the Presence of a Specific Symbiotic Bacterium SpeciesJournal of Virology, 2010
- HSP70 and Its Cochaperone CPIP Promote Potyvirus Infection in Nicotiana benthamiana by Regulating Viral Coat Protein FunctionsTHE PLANT CELL ONLINE, 2010
- A temperature sensitive mutant of heat shock protein 70 reveals an essential role during the early steps of tombusvirus replicationVirology, 2009
- Major Histocompatibility Complex Haplotype Determines hsp70-Dependent Protection against Measles Virus NeurovirulenceJournal of Virology, 2009
- Parasitization by the wasp Eretmocerus mundus induces transcription of genes related to immune response and symbiotic bacteria proliferation in the whitefly Bemisia tabaciBMC Genomics, 2008
- Vector-Virus Mutualism Accelerates Population Increase of an Invasive WhiteflyPLOS ONE, 2007
- The evolutionary and ecological role of heat shock proteinsEcology Letters, 2003
- The circulative pathway of begomoviruses in the whitefly vector Bemisia tabaci— insights from studies with Tomato yellow leaf curl virusAnnals of Applied Biology, 2002