On-pathway versus off-pathway folding intermediates
Open Access
- 1 January 1996
- journal article
- review article
- Published by Elsevier BV in Folding and Design
- Vol. 1 (1), R1-R8
- https://doi.org/10.1016/s1359-0278(96)00003-x
Abstract
Rapidly formed molten globule intermediates accumulate at the start of the folding reactions of several small proteins. Opinion is sharply divided as to whether they are on-pathway or off-pathway intermediates. I discuss recent experiments aimed at resolving this issue. Specific points include whether a 'rollover' in the plot of folding rate versus denaturant concentration implies that a folding intermediate is or is not on-pathway; whether the failure to observe folding intermediates for some small proteins implies a different folding mechanism or only that the intermediates are less stable; possible interpretation of 'fast-track' folding of hen lysozyme; and the significance of recent results in the search for unfolding intermediates.Keywords
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