Heat Stress Causes Spatially-Distinct Membrane Re-Modelling in K562 Leukemia Cells
Open Access
- 16 June 2011
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 6 (6), e21182
- https://doi.org/10.1371/journal.pone.0021182
Abstract
Cellular membranes respond rapidly to various environmental perturbations. Previously we showed that modulations in membrane fluidity achieved by heat stress (HS) resulted in pronounced membrane organization alterations which could be intimately linked to the expression and cellular distribution of heat shock proteins. Here we examine heat-induced membrane changes using several visualisation methods. With Laurdan two-photon microscopy we demonstrate that, in contrast to the enhanced formation of ordered domains in surface membranes, the molecular disorder is significantly elevated within the internal membranes of cells preexposed to mild HS. These results were compared with those obtained by anisotropy, fluorescence lifetime and electron paramagnetic resonance measurements. All probes detected membrane changes upon HS. However, the structurally different probes revealed substantially distinct alterations in membrane heterogeneity. These data call attention to the careful interpretation of results obtained with only a single label. Subtle changes in membrane microstructure in the decision-making of thermal cell killing could have potential application in cancer therapy.Keywords
This publication has 70 references indexed in Scilit:
- Targeting membrane heat-shock protein 70 (Hsp70) on tumors by cmHsp70.1 antibodyProceedings of the National Academy of Sciences of the United States of America, 2010
- Hsp70 stabilizes lysosomes and reverts Niemann–Pick disease-associated lysosomal pathologyNature, 2010
- Differential ability of cholesterol-enriched and gel phase domains to resist benzyl alcohol-induced fluidization in multilamellar lipid vesiclesBiochimica et Biophysica Acta (BBA) - Biomembranes, 2009
- Order of lipid phases in model and plasma membranesProceedings of the National Academy of Sciences of the United States of America, 2009
- Potential roles of membrane fluidity and ceramide in hyperthermia and alcohol stimulation of TRAIL apoptosisApoptosis, 2007
- Hyperfluidization-coupled membrane microdomain reorganization is linked to activation of the heat shock response in a murine melanoma cell lineProceedings of the National Academy of Sciences of the United States of America, 2007
- The hyperfluidization of mammalian cell membranes acts as a signal to initiate the heat shock protein responseThe FEBS Journal, 2005
- Condensation of the plasma membrane at the site of T lymphocyte activationThe Journal of cell biology, 2005
- Membrane fluidization triggers membrane remodeling which affects the thermotolerance in Escherichia coliBiochemical and Biophysical Research Communications, 2005
- Localization of the lipid probe 1,6-diphenyl-1,3,5 hexatriene (DPH) in intact cells by fluorescence microscopyExperimental Cell Research, 1978