TAXONOMIC REEXAMINATION OF CHARA GLOBULARIS (CHARALES, CHAROPHYCEAE) FROM JAPAN BASED ON OOSPORE MORPHOLOGY AND rbcL GENE SEQUENCES, AND THE DESCRIPTION OF C. LEPTOSPORA SP. NOV.1
- 26 August 2009
- journal article
- Published by Wiley in Journal of Phycology
- Vol. 45 (4), 917-927
- https://doi.org/10.1111/j.1529-8817.2009.00700.x
Abstract
Chara globularis Thuillier (=f. globularis sensu R. D. Wood) is a widespread species of the genus and inhabits fresh- and brackish-water environments. In an attempt to reexamine the taxonomic status of C. globularis collected from Japan, we reassessed vegetative and oospore morphology of Japanese material and herbarium specimens originating from Europe (including the type specimen) and conducted molecular phylogenetic analyses based on rbcL gene sequences. Although the other vegetative morphologies were consistent with the description of C. globularis f. globularis sensu R. D. Wood, we identified two types of branchlets within the Japanese materials: one has elongate end segments (EL type), and the other has short end segments (SH type) corresponding to the type material. Moreover, the oospore wall of the EL type was different from that in the SH type. The oospores of the EL type were dark brown to reddish brown and had a spongy pattern with the pusticular elevations on the fossa wall, whereas the fossa wall of the SH type was black with a granulate to papillate or fine pusticular pattern. In addition, our sequence data demonstrated that these two types are separated phylogenetically from each other. Therefore, we describe the EL type as a new species, C. leptospora sp. nov.Keywords
This publication has 23 references indexed in Scilit:
- Review: Taxonomy of Nitella (Charales, Charophyceae) based on comparative morphology of oospores and multiple DNA marker phylogeny using cultured materialPhycological Research, 2008
- Morphology, molecular phylogeny and taxonomy ofNitella comptonii(Charales, Characeae)Phycologia, 2006
- Phylogenetic analyses of Nitella subgenus Tieffallenia (Charales, Charophyceae) using nuclear ribosomal DNA internal transcribed spacer sequencesPhycologia, 2004
- Taxonomic re-examination of six species of Nitella (Charales, Charophyceae) from Asia, and phylogenetic relationships within the genus based on rbcL and atpB gene sequencesPhycologia, 2004
- Taxonomic re-examination of Nitella (Charales, Charophyceae) from Japan, based on microscopical studies of oospore wall ornamentation and rbcL gene sequencesPhycologia, 2002
- Monophyly of Genera and Species of Characeae based on rbcL Sequences, with Special Reference to Australian and European Lychnothamnus barbatus (Characeae: Charophyceae)Australian Journal of Botany, 1999
- Phylogeny and Biogeography of Chara (Charophyta) Inferred from 18S rDNA SequencesAustralian Journal of Botany, 1999
- Phylogeny of Extant Genera in the Family Characeae (Charales, Charophyceae) Based on rbcL Sequences and MorphologyAmerican Journal of Botany, 1996
- CHARA GLOBULARIS THUILLIER (= C. VRAGILIS DESVAUX): BREEDING PATTERNS WITHIN A COSMOPOLITAN COMPLEXLimnology and Oceanography, 1971
- NEW COMBINATIONS AND TAXA IN THE REVISION OF CHARACEAETaxon, 1962