Contribution of NADPH-oxidase to the establishment of hippocampal neuronal polarity in culture
- 1 January 2015
- journal article
- Published by The Company of Biologists in Journal of Cell Science
- Vol. 128 (16), 2989-2995
- https://doi.org/10.1242/jcs.168567
Abstract
Reactive oxygen species (ROS) produced by the NADPH oxidase (NOX) complex play important physiological and pathological roles in neurotransmission and neurodegeneration, respectively. However, the contribution of ROS to molecular mechanisms involved in neuronal polarity and axon elongation is not well understood. In this work, we found that loss of function of the NOX complex altered neuronal polarization and decreased axonal length by a mechanism that involves actin cytoskeleton dynamics. Together, these results indicate that physiological levels of ROS produced by the NOX complex modulate hippocampal neuronal polarity and axonal growth in vitro.Keywords
This publication has 37 references indexed in Scilit:
- The Light Chain 1 Subunit of the Microtubule-Associated Protein 1B (MAP1B) Is Responsible for Tiam1 Binding and Rac1 Activation in Neuronal CellsPLOS ONE, 2012
- Neuronal polarity: demarcation, growth and commitmentCurrent Opinion in Cell Biology, 2012
- The role of small GTPases in neuronal morphogenesis and polarityCytoskeleton, 2012
- The NOX toolbox: validating the role of NADPH oxidases in physiology and diseaseCellular and Molecular Life Sciences, 2012
- Direct Redox Regulation of F-Actin Assembly and Disassembly by MicalScience, 2011
- Mical links semaphorins to F-actin disassemblyNature, 2010
- The NOX Family of ROS-Generating NADPH Oxidases: Physiology and PathophysiologyPhysiological Reviews, 2007
- Genetically encoded fluorescent indicator for intracellular hydrogen peroxideNature Methods, 2006
- The Role of Local Actin Instability in Axon FormationScience, 1999
- The establishment of polarity by hippocampal neurons in cultureJournal of Neuroscience, 1988