Decomplexing biofluids using microchip based acoustophoresis
- 1 December 2008
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Lab on a Chip
- Vol. 9 (6), 810-818
- https://doi.org/10.1039/b811027a
Abstract
Highly efficient washing and extraction of microbeads to decomplex analytes ranging from small peptides to large viruses was realised in a microscaled continuous flow format. The bead washing principle reported herein is based on acoustophoresis, i.e. the primary acoustic radiation force in an ultrasonic standing wave and laminar flow properties are utilised to translate bioanalytes trapped on functionalised microbeads from one carrier fluid to another. The carry-over of non-specific material ranges from 1 to 50 ppm relative to input levels depending on application, making acoustophoresis suitable for extraction of rare species from complex environments. Selective extraction of a phosphopeptide relative to its unphosphorylated counterpart is demonstrated using metal oxide affinity capture (MOAC) beads and MALDI-TOF MS readout. Acoustophoresis of microbeads activated with specific binders could be used to capture phage viral particles. The efficiency of the acoustophoretic washing principle was demonstrated by an unspecific phage cross contamination level of only 10−6 of that in the input bead/phage mixture. The continuous flow format makes acoustophoretic washing flexible regarding sample volume and also allows for easy integration into a sequence of particle handling and analytical unit operations.This publication has 29 references indexed in Scilit:
- Polymeric integrated selective enrichment target (ISET) for solid‐phase‐based sample preparation in MALDI–TOF MSJournal of Mass Spectrometry, 2007
- On the influence of vector design on antibody phage displayJournal of Biotechnology, 2007
- Droplet‐Based DNA Purification in a Magnetic Lab‐on‐a‐ChipAngewandte Chemie-International Edition, 2006
- Carrier Medium Exchange through Ultrasonic Particle Switching in Microfluidic ChannelsAnalytical Chemistry, 2005
- Force field particle filter, combining ultrasound standing waves and laminar flowSensors and Actuators B: Chemical, 2001
- Non-destructive, non-contact handling method for biomaterials in micro-chamber by ultrasoundSensors and Actuators B: Chemical, 2000
- Matrix-assisted Laser Desorption/Ionization Mass Spectrometry Sample Preparation Techniques Designed for Various Peptide and Protein AnalytesJournal of Mass Spectrometry, 1997
- Concentration and Fractionation of Small Particles in Liquid by UltrasoundJapanese Journal of Applied Physics, 1995
- Microdialysis implemented in the design of a system for continuous glucose monitoringSensors and Actuators B: Chemical, 1993
- In vivo microdialysis - a new approach to the analysis of neurotransmitters in the brainLife Sciences, 1987